摘  要  【目的】 小菜蛾Plutella xylostella对多种化学农药产生了严重的抗药性，本研究主要为寻找防治小菜蛾新的化合物。【方法】 本研究采用4种不同极性溶剂（石油醚、二氯甲烷、乙酸乙酯和无水乙醇）提取肿柄菊Tithonia diversifolia，采用GC-MS鉴定提取物中的化合物，并评价肿柄菊提取物及其萜类成分对未交配雌性小菜蛾成虫行为的调控作用。【结果】 肿柄菊的二氯甲烷、乙酸乙酯和无水乙醇提取物对未交配雌虫具有明显的引诱作用，其中乙酸乙酯的引诱效果最为明显。肿柄菊乙酸乙酯提取物中的化学成分分析结果发现其含有植醇、α-蒎烯和角鲨烯3种萜类成分。行为实验表明，角鲨烯对小菜蛾具有驱避效果，植醇对小菜蛾具有引诱效果。田间实验结果显示，用量为1×10^﹣2 mg·mL^﹣1时，植醇对小菜蛾具有引诱效果，而角鲨烯具有驱避效果。【结论】 本研究首次证明植醇对小菜蛾行为的调节作用，其与角鲨烯是肿柄菊调节小菜蛾行为的活性成分，可以用于小菜蛾的防治。

Abstract  [Aim]  The diamondback moth, Plutella xylostella (L.) has developed high levels of resistance to several insecticides. The aim of this study was to identify a novel compound to control the diamondback moth.  [Methods]  We carried out a series of laboratory and field studies to investigate the behavioral effects of crude extract and three terpene compounds from the invasive weed Tithonia diversifolia on the diamondback moth. Crude extracts of T. diversifolia were processed in the laboratory using four different solvents, and the active compounds were identified through gas chromatography coupled with mass spectrometry. Y-tube olfactory assays were performed to evaluate the behavioral responses of unmated female diamondback moth to the crude extract and the three terpene compounds. [Results]  The results showed that unmated adult female moths were significantly attracted to crude extracts obtained using dichloromethane, ethyl acetate, and absolute ethyl alcohol. Among these, the crude extract derived from the ethyl acetate fraction demonstrated the most potent effect. Three terpene compounds, phytol, squalene and α-pinene, were identified in the crude extract. Field studies showed that phytol attracted moths at high concentrations (1×10^﹣2 mg·mL^﹣1), whereas squalene consistently repelled moths. [Conclusion]  This research identified three active terpene compounds in T. diversifolia and demonstrated the regulatory effect of phytol and squalene on diamondback moth behavior.