抗丁醚脲二斑叶螨种群筛选及生化抗性机理初探
Screening of Tetranychus urticae populations for resistance to diafenthiuron and the mechanism of biochemical resistance to this pesticide
叶调琴，杨顺义，李文平
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DOI：10.7679/j.issn.2095-1353.2024.055
作者单位：甘肃农业大学植物保护学院，甘肃省农作物病虫害生物防治工程实验室，兰州 730070
中文关键词：二斑叶螨；丁醚脲；抗性培育；解毒酶；几丁质酶
英文关键词：Tetranychus urticae; diafenthiuron; resistance breeding; detoxification enzyme; chitinase
中文摘要：
摘 要 【目的】 探究二斑叶螨Tetranychus urticae对丁醚脲的抗性发展情况及生化抗性机理，以期为二斑叶螨的抗药性治理提供理论依据。【方法】 采用室内生物测定，用丁醚脲对二斑叶螨进行持续抗性选育33代；用生化分析方法，每隔6代测定二斑叶螨丁醚脲抗性种群羧酸酯酶（Carboxylesterase，CarEs）、谷胱甘肽S-转移酶（Glutathione S-transferase，GSTs）、多功能氧化酶（Mixed-function oxidases，MFOs）和几丁质酶（Chitinases）的活力变化情况。【结果】 二斑叶螨经丁醚脲汰选至33代时，抗性指数达到18.610倍；抗性种群体内CarEs、GSTs和MFOs活性较敏感种群显著提高（P < 0.05），其相对倍数分别为1.909、2.119和7.436倍，同时几丁质酶的活性较敏感种群无显著差异（P > 0.05），其相对倍数为0.594。【结论】 二斑叶螨对丁醚脲的抗性主要与MFOs活性升高和几丁质酶活性降低有关，其次，GSTs和CarEs活性升高也参与了丁醚脲抗性的形成。
英文摘要：
Abstract [Aim] To investigate the development of resistance to diafenthiuron and the mechanism responsible for this resistance, in Tetranychus urticae. [Methods] A susceptible population of T. urticae was continuously selected for resistance to diafenthiuron for 33 generations in a laboratory. The activity of carboxylesterases (CarEs), glutathione S-transferases (GSTs), Mixed-function oxidases (MFOs) and chitinases (chitinases) in this population was tested every six generations. [Results] The resistance index had reached 18.61 after 33 generations. The activity of CarEs, GSTs and MFOs in the resistant population were significantly higher (1.909, 2.119 and 7.436-fold, respectively) than in the sensitive population (P < 0.05). However, there was no significant difference in the chitinase activity in the sensitive populations (0.594, P < 0.05). [Conclusion] Resistance of T. urticae to diafenthiuron is mainly associated with MFOs and chitinase activity, although GSTs and CarEs also play a role.