摘  要  【目的】 评价短时高温胁迫对携带球孢白僵菌Beauveria bassiana (Vuillemin)的巴氏新小绥螨Neoseiulus barkeri (Hughes)的生物学特性的影响，明确短时高温环境下巴氏新小绥螨携带球孢白僵菌联合应用防控柑橘始叶螨Eotetranychus kankitus (Ehara)的可行性。【方法】 测定35 ℃高温胁迫2、4和6 h后巴氏新小绥螨携带的球孢白僵菌Bb025菌株孢子的残留量，评价35 ℃高温胁迫4 h对携带球孢白僵菌的巴氏新小绥螨对柑橘始叶螨的捕食功能反应及其发育和繁殖的影响。【结果】 35 ℃高温短时胁迫2、4和6 h后，巴氏新小绥螨雌成螨携带的孢子数量和25 ℃不同处理时间处理后所携带的孢子数量无显著差异（c² = 0.001，P = 0.978）。尽管随处理时间的延长巴氏新小绥螨携带的孢子数量逐渐减少，但在6 h仍能达到10⁵个数量级。此外，携带球孢白僵菌的巴氏新小绥螨雌成螨在35 ℃高温胁迫4 h后对柑橘始叶螨若螨的捕食量与25 ℃处理时无显著性差异（P > 0.05），且其对柑橘始叶螨若螨的捕食潜能（a¢/T_h）小幅增加；但每日35 ℃高温胁迫4 h下，携带球孢白僵菌的巴氏新小绥螨发育加快、产卵前期延长、产卵量和寿命降低。【结论】 短时高温环境下，巴氏新小绥螨仍可携带大量的球孢白僵菌孢子，携带球孢白僵菌的巴氏新小绥螨对柑橘始叶螨具有较好的防控能力，但短时高温对携带球孢白僵菌的巴氏新小绥螨的生殖和寿命等方面存在不利影响。本研究结果对研发菌螨协同联合应用的可持续性综合防控柑橘害螨的策略具有一定指导的意义。

Abstract  [Aim]  To determine the effect of short-term high temperature stress on the biological characteristics of Neoseiulus barkeri (Hughes) carrying Beauveria bassiana (Vuillemin). The findings of this study will determine the feasibility of using N. barkeri carrying B. bassiana for the control of Eotetranychus kankitus (Ehara) in short-term high temperature environments. [Methods]  The residual amount of B. bassiana conidia carried by N. barkeri was counted following exposure to high-temperature stress at 35 ℃ for 2, 4, and 6 hours. We evaluated the functional response of B. bassiana-carrying N. barkeri in controlling E. kankitus, and its effects on development and fecundity following exposure to high-temperature stress at 35 ℃ for 4 hours. [Results]  After 2, 4, and 6 hours of short-term high temperature stress at 35 ℃, there was no significant difference in the number of conidia carried by female N. barkeri compared to 25 ℃ (c² = 0.001, P = 0.978). The number of conidia carried gradually decreased over time. However, one female N. barkeri still carried 10⁵ conidia after 6 hours. Additionally, after 4 hours of high-temperature stress at 35 ℃, there was no significant change in the predation rate of B. bassiana-carrying N. barkeri female on E. kankitus nymphs compared to the rate at 25 ℃ (P > 0.05), although the predation potential (a¢/T_h) did increase slightly. However, when exposed to high-temperature stress at 35 ℃ for 4 hours per day, B. bassiana-carrying N. barkeri exhibited accelerated development, a prolonged pre-oviposition period, reduced fecundity, and a decreased lifespan. [Conclusion]  Under short-term high temperature conditions, N. barkeri can carry a substantial amount of B. bassiana conidia. Furthermore, when N. barkeri carries B. bassiana, it is still able to effectively control E. kankitus. However, it is important to note that exposure to short-term high temperature stress negatively impacts the reproduction and lifespan of N. barkeri carrying B. bassiana. This study provides valuable insights for the development of sustainable and integrated strategies to control citrus pest mites by combining the use of fungi and predatory mites.