三叶斑潜蝇地理种群间的耐热性及 Hsp64.9表达研究
Differences in thermal tolerance and the expression of Hsp64.9 between geographical populations of Liriomyza trifolii
常亚文1 仲 玲1 闫玉卿1 赵婧雅1 龚伟荣1, 2 杜予州1, 3
点击：341次 下载：37次
DOI：10.7679/j.issn.2095-1353.2024.095
作者单位：1. 扬州大学植物保护学院暨应用昆虫研究所；2. 江苏省植物保护植物检疫站；3. 教育部农业与农产品安全国际合作联合实验室
中文关键词：三叶斑潜蝇；地理种群；高温处理；耐热性；Hsp64.9
英文关键词：Liriomyza trifolii; geographical population; high temperature; heat tolerance; Hsp64.9
中文摘要：【目的】 明确三叶斑潜蝇Liriomyza trifolii不同地理种群的高温耐受性和重要热激蛋白基因Hsp64.9的表达模式，探究三叶斑潜蝇地理种群间温度适应性差异的机制。【方法】 以三叶斑潜蝇海南和江苏种群为研究对象，分别测定其成虫和蛹在不同高温条件（41-46 ℃，温度梯度1 ℃）胁迫1 h和同一温度不同胁迫时长处理（39、42和45 ℃，处理时长0.25、0.5、1、2和4 h）下的存活率和羽化率，并比较基因Hsp64.9的表达差异。【结果】 江苏和海南种群成虫的存活率分别在42和43 ℃开始显著降低（江苏种群：F6,14=296.459，P<0.001；海南种群：F6,14=255.810，P<0.001），且不同温度条件下，海南种群成虫的存活率显著高于江苏种群（P<0.05）。在39 ℃胁迫时长短于4 h及 42和45 ℃胁迫时长短于1 h处理下，2个种群成虫的存活率无显著差异（P>0.05），随着胁迫时长的延长，海南种群成虫存活率显著高于江苏种群（P<0.05），除了存活率为0的45 ℃下2和4 h处理。海南种群蛹的羽化率在42、44和46 ℃显著高于江苏种群（42 ℃：t=﹣4.234，P=0.013；44 ℃：t=﹣5.567，P=0.005；46 ℃：t=﹣4.000，P=0.016）。同一温度不同胁迫时长处理下，除了42 ℃胁迫时长1 h外（t=-4.243，P=0.013），2个种群蛹的羽化率均没有显著差异（P>0.05）。海南种群成虫和蛹在不同温度及同一高温不同胁迫时长处理下，除了成虫44 ℃处理1 h（t=﹣0.475，P=0.659）和蛹45 ℃处理0.5 h（t=2.723, P=0.053）外，基因Hsp64.9的相对表达量均显著高于江苏种群。【结论】 三叶斑潜蝇不同地理种群间的耐热性存在显著差异，Hsp64.9的差异表达可能是海南种群耐热性高于江苏种群的原因。
英文摘要：
[Aim] To investigate differences in the
high temperature tolerance and expression of the heat shock protein gene Hsp64.9 between different geographical
populations of Liriomyza trifolii,
and investigate the mechanism of temperature adaptation in this species. [Methods] Adults and pupae from the Hainan and Jiangsu
populations of L. trifolii were
exposed to different high temperatures for different periods. Differences in
heat tolerance between these geographical populations were then assessed by
comparing adult survival rates and pupal emergence rates. The expression of Hsp64.9 in adults and pupae from each
population were also measured and compared. [Results] The survival rate of both the Jiangsu and
Hainan populations began to decrease significantly at 42 and 43 ℃ (Jiangsu population: F6,14=296.459, P<0.001; Hainan population: F6,14=255.810, P<0.001), respectively, but the
survival rate of the Hainan population was significantly higher than that of
the Jiangsu population (P<0.05).
There was no significant difference in survival rate between the two
populations following exposure to 39 ℃ for
less than 4 h, or exposure to 42 or 45 ℃ for
less than 1 h (P>0.05). The
survival rate of adults from the Hainan population was significantly higher
than that of those from the Jiangsu population following longer durations of
exposure to high temperatures (P<0.05).
The emergence rate of the Hainan population was also significantly higher than
that of Jiangsu population after exposure to 42, 44
and 46 ℃ for 1 h (42 ℃: t=﹣4.234, P=0.013;
44 ℃: t=﹣5.567, P=0.005;
46 ℃: t=﹣4.000, P=0.016),
but duration of exposure to high temperature had no significant effect on the
emergence rate of pupae from these populations (P>0.05). The expression of Hsp64.9 in adults and pupae from the Hainan population was significantly higher
than that of those from the Jiangsu population under all treatment combinations
(P<0.05). [Conclusion] The Hainan population of L. trifolii is significantly more tolerant to high temperatures
than the Jiangsu population, an adaptation that, at least in part, could be due
to greater expression of the Hsp64.9 gene.