不同浓度噻虫嗪对点蜂缘蝽若虫体内 保护酶和解毒酶活性的影响
Effects of different concentrations of thiamethoxam on the activitiy of protective and detoxification enzymes in Riptortus pedestris
王自杰1, 2 杨芳哲2 柳添杰2 田鑫月2 王月英3 史树森2 高 宇
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DOI：10.7679/j.issn.2095-1353.2024.105
作者单位：1. 黑龙江省农业科学院佳木斯分院，农业部佳木斯作物有害生物科学观测实验站； 2. 吉林农业大学植物保护学院，农业农村部大豆病虫害防控重点实验室； 3. 宿州市农业科学院
中文关键词：点蜂缘蝽；噻虫嗪；保护酶；解毒酶；抗药性
英文关键词：Riptortus pedestris; thiamethoxam; protective enzymes; detoxification enzymes; drug resistance
中文摘要：【目的】 探明不同浓度噻虫嗪胁迫点蜂缘蝽Riptortus pedestris F0代3龄若虫后，其体内和F1代3龄若虫体内保护酶和解毒酶活性的变化。【方法】 采用药液浸渍法，将清水浸成的“肾型”大豆籽粒，浸入25%噻虫嗪水分散粒剂（Water dispersible granule，WG）用清水配置成LC10（5.2 mg/L）、LC20（11.2 mg/L）、LC30（19.6 mg/L）、LC40（31.6 mg/L）和LC50（49.4 mg/L）药液20 s，设清水对照处理。喂食点蜂缘蝽F0代3龄若虫 24 h后，分别将各处理存活的若虫，一部分测定其体内过氧化物酶（Peroxidase，POD）、过氧化氢酶（Catalase，CAT）、超氧化物歧化酶（Superoxide dismutase，SOD）、乙酰胆碱酯酶（Acetylcholinesterase，AChE）、谷胱甘肽转移酶（Glutathione S-transferase，GST）和羧酸酯酶（Carboxylesterase，CarE）的酶活性变化；另一部分放入养虫笼中喂食清水处理的大豆籽粒，饲养至F1代若虫发育至3龄时，测定其体内POD、CAT、SOD、AChE、GST和CarE的酶活性变化。【结果】 不同浓度噻虫嗪处理后，F0代若虫体内POD、CAT、SOD、GST及CarE活性均高于对照组；F1代若虫体内POD、AChE、GST和CarE的酶活性高于对照组。所有浓度处理下，点蜂缘蝽F1代若虫体内POD、GST和CarE的酶活性均高于F0代；在LC20-LC50浓度处理，点蜂缘蝽F1代若虫体内AChE的酶活性均高于F0代。点蜂缘蝽F0和F1代若虫体内保护酶和解毒酶的活性与噻虫嗪浓度呈正相关。【结论】 在噻虫嗪胁迫下，F0代若虫通过提高体内POD、CAT、SOD、GST和CarE的酶活性进行解毒代谢，F1代若虫则通过提高POD、AChE、GST和CarE活性进行解毒代谢。POD、AChE、GST和CarE酶活性的增加可能是点蜂缘蝽F1代若虫产生抗药性的主要原因。
英文摘要：
[Aim] To measure changes in the activity of protective and detoxifying
enzymes in the F0 and the F1 generations of the 3rd
instar of Riptortus pedestris nymphs exposed to different concentrations
of thiamethoxam. [Methods] Reniform
soybean seeds soaked in water were immersed in 25% thiamethoxam WG that had
been diluted with different amounts of water to obtain LC10 (5.2
mg/L), LC20 (11.2 mg/L), LC30 (19.6 mg/L), LC40 (31.6 mg/L) and LC50 (49.4 mg/L) solutions. Water was the control. F0 nymphs of R. pedestris were fed the treated seeds for 24 hours after
which changes in the activity of peroxidase (POD), catalase (CAT), superoxide
dismutase (SOD), acetylcholinesterase (AChE), glutathione S-transferase
(GST) and carboxylesterase (CarE) in surviving nymphs was measured. Another
group of nymphs was placed in cages and fed soybean seeds treated with water
until F1 generation nymphs had developed to the 3rd instar, after
which changes in the activity of POD, CAT, SOD, AChE, GST, and CarE were
determined in vivo. [Results] After
treatment with different concentrations of thiamethoxam, the POD, CAT, SOD, GST
and CarE activity of F0 generation nymphs was higher than that of
the control group. POD, AChE, GST and CarE activity of F1 larvae
were also higher than that of the control group. The POD, GST and CarE activity
of F1 larvae was higher than those of F0 larvae for all
treatment concentrations. AChE enzyme activity of F1 larvae was
higher than that of F0 larvae for concentrations in the range of LC20-LC50.
Protective and detoxifying enzyme activity in F0 and F1 larvae was positively correlated with thiamethoxam concentration. [Conclusion]
F0 R. pedestris nymphs detoxify thiamethoxam by increasing their POD, CAT, SOD, GST and CarE
enzyme activity, whereas F1 nymphs detoxify thiamethoxam by
increasing their POD, AChE, GST and CarE activity. The observed changes in POD,
AChE, GST and CarE enzyme activity may be the mechanism responsible for
pesticide resistance in R. pedestris F1 nymphs.