蓼蓝齿胫叶甲肠道微生物多样性研究
Diversity of the gut microbiota of Gastrophysa atrocyanea
王 毛1 贺 宏2 张云书2 肖 敏3 魏真真1 郭静微1 陈 松1 陈庆东
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DOI：10.7679/j.issn.2095-1353.2024.109
作者单位：1. 四川省农业科学院植物保护研究所，农业部西南作物有害生物综合治理重点实验室； 2. 阿坝藏族羌族自治州农业科学技术研究所；3. 阿坝州生态保护与发展研究院； 4. 阿坝州农产品质量检验监测中心；5. 阿坝州植保植检站
中文关键词：蓼蓝齿胫叶甲；酸模；唐古特大黄；肠道微生物；天敌
英文关键词：Gastrophysa atrocyanea; Rumex acetosa; Rheum tanguticum; intestinal microbiota; natural enemies
中文摘要：
【目的】 明确蓼蓝齿胫叶甲Gastrophysa atrocyanea取食酸模Rumex acetosa和唐古特大黄Rheum tanguticum后肠道微生物多样性的特点。【方法】 通过高通量测序技术，分析取食酸模和唐古特大黄的蓼蓝齿胫叶甲体内肠道微生物种类、丰度、优势度、多样性以及功能变化。【结果】 蓼蓝齿胫叶甲取食酸模和唐古特大黄后肠道微生物共有154种，其中取食酸模的独有种548种，取食唐古特大黄的独有种554种；变形菌门是这两组蓼蓝齿胫叶甲肠道微生物的优势群；取食唐古特大黄的第3个样本肠道微生物物种多样性最高；代谢功能预测物种组成结果表明，除沙雷氏菌，取食酸模的优势群为泛菌Pantoea，取食唐古特大黄的优势群为肠杆菌科。【结论】 本研究初步明确了蓼蓝齿胫叶甲取食酸模和唐古特大黄后的肠道微生物多样性特点，为其寄主范围和种群暴发预测提供了一定的理论依据。
英文摘要：
[Aim] Gastrophysa atrocyanea is an insect that feeds on weeds of the family Polygonaceae. [Methods] High- throughput
sequencing analysis was used to investigate the type, abundance, dominance and
diversity of, and functional changes in, the gut microbiota in G. atrocyanea that fed on either Rumex acetosa or Rheum tanguticum. [Results] A total of
154 gut microbiota species were identified, of which 548 were unique to G.
atrocyanea that fed on R. acetosa and 554 to those that fed on R. tanguticum. The Proteobacteria was
the dominant gut microbiota in both treatment groups. Species diversity was
highest in G. atrocyanea that had fed on R. tanguticum. The species composition predicted from the results
of a metabolic function analysis indicate that, except for Serratia, the
dominant group in the R. acetosa treatment group was Pantobacterium, and
the dominant group in the R. tanguticum treatment group was Enterobacterium. [Conclusion] This study preliminarily
clarified the diversity characteristics of gut microbiota of G. atrocyanea that feed on R. acetosa and R. tanguticum, providing a
theoretical basis for its host range and population outbreak prediction.