【目的】 产卵是昆虫行为的重要环节之一，观察产卵行为可为进一步探索害虫防治及益虫保护措施提供基础数据。【方法】 本研究利用工业CCD（Charge coupled device）视频显微镜和红外光源作为拍摄器材，以自主研制的昆虫产卵装置观察双斑长跗萤叶甲Monolepta signata 的产卵行为。【结果】 通过观察双斑长跗萤叶甲产卵在不同产卵介质的产卵情况，发现白沙较为适合作为产卵介质；双斑长跗萤叶甲雌成虫在白沙上产卵过程可分为6个阶段：靠近寄主、接触试探、刺探识别、产卵、产卵器收回和静止。其中第3阶段雌成虫利用腹部末端刺探识别过程时间最长，产卵时身体腹面与产卵介质表面的夹角、产卵深度和腹部末端伸长长度三者有一定正相关性。【结论】 通过CCD视频显微镜、红外光源和自主研制的产卵观察装置组合能清楚观察双斑长跗萤叶甲在土中的产卵过程，为观察其他昆虫在土中产卵行为提供新方法，也为进一步探索昆虫产卵行为并利用其生物学特性，对在土中产卵的植食性昆虫的防治提供技术支持。

[Aim]  Oviposition is a crucial aspect of insect behavior, and observing oviposition behavior can provide fundamental data for further exploration of pest control and the conservation of beneficial insects. [Methods]  An industrial CCD video microscope paired with an infrared light source were used to record the oviposition behavior of Monolepta signata in a custom-built insect oviposition chamber. Additionally, different substates were used to assess the oviposition preference of M. signata. [Results]  The highest oviposition rate was observed when white sand was used as a substrate. The oviposition process of M. signata on white sand was categorized into six stages: Host approach, contact probing, probing identification, oviposition, retrieving the ovipositor, and stationary. The probing identification stage was the longest, with females using the end of their abdomen to probe and identify oviposition sites. A positive correlation was observed between the angle of the female abdomen and the substrate surface, and the depth of oviposition and the length of the abdominal tip during oviposition. [Conclusion]  The combination of a CCD video microscope, infrared light source, and a custom-built oviposition observation chamber enabled clear observation of the oviposition process of M. signata in the soil. This study introduces a novel approach for observing insect oviposition behavior, furthering the investigation of their biological traits for practical applications. It also offers technical support for the control of phytophagous insects that oviposit in the soil.