【目的】 明确茄科植物曼陀罗次生代谢物质阿托品对西花蓟马Frankliniella occidentalis雌成虫体内保护酶和解毒酶活性的影响。【方法】 通过夹膜饲喂法对西花蓟马雌成虫进行生物毒力测定，计算得到25%、50%和75%致死浓度相应的浓度，分别作为低浓度、中浓度和高浓度；用低、中、高3种浓度阿托品溶液饲喂胁迫西花蓟马雌成虫，测定饲喂12、24和48 h后，西花蓟马体内3种保护酶[过氧化氢酶(Catalase，CAT)、超氧化物歧化酶(Superoxide dismutase，SOD)和过氧化物酶(Peroxidase，POD)]与3种解毒酶[谷胱甘肽硫转移酶(Glutathione S-transferase，GST)、羧酸酯酶(Carboxylesterase，CarE)、昆虫细胞色素P450(Cytochrome P450，CYP450)]的活性。【结果】 阿托品对西花蓟马雌成虫体内保护酶和解毒酶均产生了显著影响，不同浓度阿托品胁迫显著提高了西花蓟马雌成虫体内3种保护酶CAT、SOD和POD活性（CAT: P＜0.001；SOD: P＜0.001；POD: P＜0.001），并呈现出与胁迫浓度正相关的趋势，同时均在48 h酶活性达到最高值，尤其是SOD和POD活性在高浓度下的升幅最大；3种浓度阿托品胁迫诱导西花蓟马解毒酶GST和CYP450酶活性，先迅速增强而后下降，分别在12和24 h达到峰值，CarE酶活性仅在低浓度胁迫12 h显著增强（P＜0.001），中、高浓度下活性则持续被抑制。【结论】 西花蓟马雌成虫体内保护酶和解毒酶活性在阿托品胁迫下发生了明显的变化，说明西花蓟马通过调节保护酶和解毒酶适应植物次生代谢物质阿托品的影响。

 [Aim]  To investigate the effects of atropine, a secondary metabolite of Datura stramonium, on protective and detoxification enzyme activity in adult, female Frankliniella occidentalis. [Methods]  Using the membrane feeding method, the bioassay for toxicity of F. occidentalis female adults was conducted, and the 25%, 50%, and 75% lethal concentrations were calculated and designated as low, medium, and high concentrations, respectively. Female adults were then exposed to atropine solutions at these three concentrations via membrane feeding. The activity of three protective enzymes [catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD)], and three detoxification enzymes [glutathione S-transferase (GST), carboxylesterase (CarE), and cytochrome P450 (CYP450)], were then determined in each treatment group after 12, 24 and 48 h. [Results]  The activity of both protective and detoxification enzymes were significantly affected by atropine dosage. Protective enzyme (CAT, SOD, and POD) activity significantly increased in a concentration-dependent manner (CAT: P < 0.001; SOD: P < 0.001; POD: P < 0.001), with peak activity observed at 48 h. Notably, increases in SOD and POD activity were most pronounced in the high concentration treatment group. With respect to detoxification enzymes, GST and CYP450 activity initially increased, then decreased, peaking at 12 and 24 h, respectively. CarE activity was significantly enhanced only in the low-concentration group at 12 h (P < 0.001), but was continuously inhibited in the medium and high concentration groups. [Conclusion]  The activity of protective and detoxification enzymes in adult female F. occidentalis were significantly affected by atropine stress, which indicates that females of this species adapt to the effects of this plant secondary metabolite by regulating their protective and detoxification enzyme activity.