【目的】 探究茶园生境中两种主要叶甲害虫柳蓝叶甲Plagiodera versicolora和蓝胸圆肩叶甲Humba cyanicollis成虫的肠道微生物组成和功能。【方法】 基于微生物扩增子测序技术，分析两种叶甲肠道细菌与真菌的群落特征和功能。【结果】 两种叶甲的肠道细菌优势门均为变形菌门Proteobacteria和厚壁菌门Firmicutes，主要优势属为肠杆菌属Enterobacter、内共生体Endosymbionts、螺原体属Spiroplasma、乳球菌属Lactococcus等；肠道真菌优势门均为子囊菌门Ascomycota和担子菌门Basidiomycota，主要优势属为枝孢霉属Cladosporium、镰刀菌属Fusarium、青霉属Penicillium、曲霉属Aspergillus、亚隔孢壳属Didymella等。两种叶甲的主要肠道细菌和真菌类群丰度存在差别，但多样性无明显差异，细菌的群落结构差异显著（P=0.001）而真菌的无明显差异。两种叶甲的共有细菌和真菌类群构成了肠道微生物的主体。同时，功能分析表明两种叶甲的肠道细菌和真菌的功能类型均差别不大。【结论】 本研究揭示了茶园生境的柳蓝叶甲和蓝胸圆肩叶甲的肠道微生物的组成、群落结构以及功能特征，为茶园鞘翅目叶甲类害虫的生物防治提供了参考依据。

 [Aim]  To characterize the composition and potential functions of the gut microbiota of two major leaf beetle pests, Plagiodera versicolora and Humba cyanicollis, in tea plantation ecosystems. [Methods]  Microbial amplicon sequencing was used to analyze gut microbiota community structure and predict the functions of the gut bacteria and fungi of both species. [Results]  The gut bacterial communities of both species were dominated by Proteobacteria and Firmicutes at the phylum level, with key genera including Enterobacter, endosymbionts, Spiroplasma, and Lactococcus. The fungal communities were primarily composed of Ascomycota and Basidiomycota, dominated by Cladosporium, Fusarium, Penicillium, Aspergillus, and Didymella. Although the relative abundance of major microbial taxa differed between beetle species, there were no significant differences in alpha diversity. Although the bacterial community structure (beta-diversity) was significantly different between species (P=0.001), fungal community structure was not. A core microbiota, consisting of shared bacterial and fungal taxa, formed the majority of the gut microbial community in both species. Furthermore, functional prediction indicated minimal differences in the metabolic potential of both bacteria and fungi between the two species. [Conclusion]  These findings describe the structure and potential functions of the gut microbiota of P. versicolora and H. cyanicollis, providing a valuable foundation for developing targeted biological control strategies for these coleopteran tea pests.