上海地区甜菜夜蛾田间种群对三种杀虫剂的抗药性和抗性基因突变检测
Monitoring insecticide resistance and detecting resistant gene mutations in Spodoptera exigua (Lepidoptera: Noctuidae) populations in Shanghai
段瑞冠1, 2** 梅国红3 陈义娟2 张 浩2 王金彦2 蒋杰贤2 季香云2***
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DOI：10.7679/j.issn.2095-1353.2026.066
作者单位：1. 上海海洋大学水产与生命学院，上海 201306；2. 上海市农业科学院生态环境保护研究所，上海 201403； 3. 上海市金山区农业技术推广服务中心，上海201599
中文关键词： 甜菜夜蛾；抗药性检测；基因突变
英文关键词： Spodoptera exigua; resistance monitoring; gene mutation
中文摘要：
【目的】 明确上海地区甜菜夜蛾Spodoptera exigua对常用杀虫剂的敏感性，为科学防控该虫提供科学依据。【方法】 采用饲料药膜法，测定上海郊区（奉贤区、金山区及嘉定区）甜菜夜蛾田间种群对溴氰虫酰胺、甲氨基阿维菌素苯甲酸盐和乙基多杀菌素共3种杀虫剂的敏感性；运用PCR技术检测该虫田间种群鱼尼丁受体RyR、乙酰胆碱酯酶受体nAChRα6和细胞色素P450酶CYP9A186的抗性基因突变位点的突变频率。【结果】 金山区甜菜夜蛾种群对溴氰虫酰胺与甲氨基阿维菌素苯甲酸盐均呈现出高等抗性水平，而奉贤与嘉定区种群则表现为中等抗性水平；3个种群对乙基多杀菌素均保持较高敏感性。甜菜夜蛾鱼尼丁受体SeRyR基因I4743M位点的抗性等位基因频率介于92.0%-100.0%之间，未发现G4900E突变；CYP9A186基因F116V位点的抗性等位基因频率为75.0%-96.0%，而乙酰胆碱酯酶受体nAChR α6基因G275E位点的抗性等位基因频率则处于14.0%-26.0%之间。【结论】 上海地区甜菜夜蛾对溴氰虫酰胺和甲氨基阿维菌素苯甲酸盐的敏感性较低，对乙基多杀菌素的敏感性较高。采用药剂轮换或选用无交互抗性药剂是区域性防控甜菜夜蛾的重要措施。
英文摘要：
[Aim] To clarify the susceptibility of Spodoptera exigua in
Shanghai to commonly used insecticides and provide a scientific basis for its
control. [Methods] The
diet-overlay method was used to determine the susceptibility of wild
populations of S. exigua in suburban Shanghai (Fengxian, Jinshan, and
Jiading districts) to three insecticides (cyantraniliprole, emamectin benzoate,
and spinetoram). PCR technology was used to detect the mutation frequencies of
resistance-related gene loci in these populations, including the ryanodine
receptor (RyR), the nicotinic acetylcholine receptor (nAChRα6), and the
cytochrome P450 enzyme (CYP9A186). [Results] The Jinshan population had a high-level of
resistance to both cyantraniliprole and emamectin benzoate, whereas the
Fengxian and Jiading populations had moderate resistance to these two
insecticides. All three populations remained highly susceptible to spinetoram.
The frequency of resistant alleles at the I4743M site of the SeRyR gene ranged
from 92.0% to 100.0%. The G4900E mutation was not detected. The frequency of
resistant alleles at the F116V site of the CYP9A186 gene ranged from 75.0% to
96.0%, whereas that at the G275E site of the nAChR α6 gene ranged from 14.0% to
26.0%. [Conclusion] Spodoptera
exigua populations in Shanghai have moderate to high resistance to
cyantraniliprole and emamectin benzoate but remain susceptible to spinetoram.
Rotating the use of insecticides, or using non-cross-resistant insecticides,
could be crucial for the effective management of S. exigua in this
region.