中国二化螟不同地理种群的AFLP遗传分析
Genetic diversity of different geographical populations of Chilo suppressalis (Walker) in China by AFLP
韩光杰，罗光华2，张志春2，王利华2
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作者单位：江苏省农科院、南京农业大学
中文关键词：二化螟；地理种群；AFLP；遗传多态性
英文关键词：Chilo suppressalis; geographic population; AFLP; genetic diversity
中文摘要：利用AFLP标记对中国11省13个二化螟地理种群进行遗传多态性分析，以揭示不同区域种群间遗传分化，为建立较准确的二化螟发生区划及区域治理对策提供新的依据。选取3对引物，共扩增出445个位点，其中多态性位点数为386，多态性比率为86.8%。种群间分化系数（Gst）为0.74，基因流仅为0.176；13个地理种群间遗传一致度较高，均大于0.67。遗传距离聚类分析表明，二化螟13个地理种群可区分为4类，其中淮北平原类（赣榆、阜宁、阜南）、东南山区类（鄞县、闽侯）、荆湘川类（荆州、邵阳、德阳）三类种群分别具有较一致的地理气候特征或相对较近的距离。研究还表明，与其他测试种群的遗传距离普遍较大、相对孤立的地理种群，其聚类结果有一定的随机性，因此，取样点较多、且样点分布较均匀的聚类分析结果才有较高可信度。
英文摘要：DNA molecular markers by amplified fragment length polymorphism (AFLP) were applied to detect the genetic variation of 13 geographical populations of Chilo suppressalis collected from 11 provinces of China. A toltal of 445 distinct alleles were amplified by three AFLP primer pairs from C. suppressalis genomic DNA, of which 386 alleles (86.8%) were found to be polymorphic. The coefficient of gene differentiation between the populations was 0.74. There was high genetic identity between the 13 populations with the value greater than 0.67. Cluster analysis showed that all the tested populations could be grouped into 4 clades based on polymorphism of AFLP markers, of which the northern Huai river plain clade including GY, FN and FNing populations, the east-south region clade including YX and MH populations, and the Jing-Xiang-Chuang plain clade including JZ, SY and DY populations are credible in that the populations within each of the clades are consistently subject to similar geographic climate and near distance. However, the lone population with longer genetic distance to all other tested populations was probably clustered into a random clade. So the sampled populations for genetic polymorphism clustering should be as many as possible and dispersed