蜜蜂羽化温度与卷翅病毒动态复制相关性研究
The correlation between bee (Apis mellifera) emergence temperature and replication dynamics of the deformed wing virus
张炫1，2**陈彦平2周丹银1和绍禹1
点击：1636次 下载：14次
DOI：
作者单位：1. 云南农业大学东方蜜蜂研究所昆明650201；2.美国农业部东部蜜蜂研究室马里兰州20705
中文关键词：西方蜜蜂， 卷翅病毒， 羽化温度， 病毒浓度
英文关键词：Apis mellifera, deformed wing virus, emergence temperature, virus titer
中文摘要：
病毒复制动态变化对于病毒病的研究有着重要意义。选用蜜蜂卷翅病毒（deformed wing virus，以下简称 DWV）作为研究模型，检测DWV隐性感染蜂群在不同温度条件下羽化工蜂体内的病毒浓度，以了解不适温度压力这一非生物因子对蜜蜂羽化率及蜜蜂体内DWV 病毒复制动态的影响。实验结果显示不适羽化温度不仅会明显降低蜜蜂羽化成功率，而且新羽化的带毒蜜蜂体内DWV病毒拷贝量增加极显著。部分解释了冬季和早春低温环境下因病毒病暴发造成人工饲养蜂群非正常损失的原因。
英文摘要：Understanding the replication dynamics of viruses is vital to the study of disease pathogenesis. The dynamics of the deformed wing virus (DWV) in honeybee hosts was investigated. Pupae from a colony with no apparent DWV infection were bred at different temperatures. Newly emerged worker bees were collected and virus titers calculated. We found that unsuitable emergence temperatures cause a sharp decline in adult emergence from pupae. Simultaneously, DWV titers significantly increased in the adults that did emerge. These results partly explain abnormal colony losses during periods of low temperature in winter and early spring. Our laboratory studies support the correlation between abiotic stress factors and the dynamics of virus replication in honey bees.