透射电镜树脂包埋块中昆虫感受器的定位技术

Microtomy of insect sensilla embedded in resin blocks for transmission electronic microscopy

张燕如,任利利,骆有庆

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作者单位：北京林业大学省部共建森林培育与保护教育部重点实验室北京100083

中文关键词：昆虫感受器，荧光显微镜，扫描电镜，透射电镜，树脂包埋块

英文关键词：insect sensilla, fluorescence microscope, scanning electronic microscopy, transmission electron microscope , resin block

中文摘要：

运用透射电子显微镜技术，可以对昆虫的不同器官上的感受器进行切片观察，以揭示感受器内部的清晰构造，为分析感受器功能提供依据。但目前在切片技术中，准确定位不同类型的感受器存在困难。通过将莱卡md2500荧光显微镜与日立S\|3400N扫描电镜结合，确定透射电镜树脂包埋块中的昆虫感受器位置，提高运用透射电镜观察昆虫感受器超微结构时切片的准确性。

 

英文摘要：Transmission electronic microscopy (TEM) can be used to examine microslices of sensilla located on different insect organs thereby revealing the ultrastructure of each type of sensilla and providing evidence for their function analysis. However, it’s difficult to accurately locate different types of sensilla by mocrotomy for TEM. The precise location of insect sensilla embedded inside resin blocks was determined using both scanning electronic microscopy (HACHI S3400N) and fluorescent microscopy (Leica md2500). This new method of microtomy of insect samples in resin blocks can improve the accuracy of locating insect sensilla and save research costs.

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