墨天牛属昆虫COⅠ基因比较及系统学初步研究
Sequence comparison and phylogenetic analysis of mtDNA CO Igene sequence fragments in Monochamus species(Coleoptera: Cerambycidae)
郑斯竹1** 张 凯2*** 杨晓军3 徐 梅3 安榆林3
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DOI：10.7679/j.issn.2095-1353.2015.041
作者单位：1. 苏州出入境检验检疫局外来有害生物防控技术中心，苏州215000；2. 上海共青森林公园，上海200000；3. 江苏出入境检验检疫局，南京210001
中文关键词：墨天牛属，线粒体COⅠ基因，遗传距离，系统发育树
英文关键词： Monochamus, mtDNACOⅠgene, genetic distance, phylogenetic tree
中文摘要：
【目的】 对11种墨天牛线粒体DNA细胞色素氧化酶C亚基Ⅰ基因（COⅠ）进行比较并对墨天牛属系统发育关系进行初步探讨。【方法】 本文测定分析了11种墨天牛线粒体DNA细胞色素氧化酶C亚基Ⅰ基因（COⅠ），并采用简约法和贝叶斯推论法构建了墨天牛属的分子进化树。【结果】 序列比对分析得到470 bp大小的COⅠ基因片段，其中可变异位点169个（36.0%），保守位点301个（64.0%），转换/颠换的平均值（R值）为1.03，说明此段序列适合于分子进化树。利用不同系统发育重建方法得到的进化树具有相似的拓扑结构，同时结合形态学分类特征对墨天牛属昆虫的分子系统进化关系进行探讨。结果显示分子结果与形态分类结果相似。【结论】 利用COⅠ基因构建的墨天牛属系统发育树是探讨墨天牛分类的有效方法。
英文摘要：
To determine the molecular phylogeny of Monochamus spp. by analyzing variation in mitochondrial COⅠgene sequences. [Methods] Mitochondrial COⅠgene sequences from 11 species were analyzed using MEGA 5.0. Phylogenetic trees for Monochamus were constructed using both the Parsimony (MP) and Bayesian-Inference (BI) methods. [Result] Sequence analysis results indicate that, after alignment, 470 sites consisted of 169 variable sites (36.0%) and 301 conserved sites (64.0%). The transition versus transversion ratio (R-value) was 1 .03. Saturation plots indicate that combined analysis had better a linear relationship than the single gene analysis, suggesting that base mutations have not reached saturation. Phylogenetic trees based on MP and BI methods had similar topologies, which was consistent with the results of the traditional morphology. Our results support the taxonomic validity of the genus Monochamus. [Conclusion] This study confirms that sequence analysis is an effective method for studying the taxonomy of Monochamus.