白背飞虱气味结合蛋白SfOBP11与寄主选择行为的相关性研究
The relationship between odorant binding proteins and host locatingbehavior in Sogatella furcifera (Hemiptera: Delphacidae)
蒋艳冬** 梁庆梅 白月亮 周文武 刘 苏 王光华*** 祝增荣***
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DOI:10.7679/j.issn.2095-1353.2016.061
作者单位:水稻生物学国家重点实验室,农业部农业昆虫学重点实验室,浙江大学昆虫科学研究所,杭州 310058
中文关键词:白背飞虱,气味结合蛋白,寄主选择,RNA干扰
英文关键词:the white-backed planthopper, olfactory, host locating behavior, RNAi
中文摘要:
【目的】 研究白背飞虱Sogatella furcifera气味结合蛋白的生物学功能,筛选与寄主植物气味感知过程相关的关键基因,为研发基于RNAi的白背飞虱防控策略提供理论依据。【方法】 利用显微注射法对触角中特异性高表达的SfOBP11进行RNA干扰,并使用实时荧光定量PCR检测干扰效率,最后采用H型嗅觉选择仪观察白背飞虱对寄主水稻识别能力的变化。【结果】 SfOBP11 mRNA相对表达水平在注射48 h后显著下降,但试虫的存活率未受影响。注射SfOBP11 dsRNA的若虫对寄主水稻的识别能力降低。【结论】 SfOBP11可能在白背飞虱识别寄主水稻挥发物的过程中发挥重要作用。
英文摘要:
[Objectives] To provide a basis for effective, RNA interference (RNAi) -based, pest control, the biological functions of the odorant binding proteins of Sogatella furcifera (Hemiptera: Delphacidae) were investigated and the genes involved in host-locating behavior were identified. [Methods] SfOBP11 was knocked down by RNAi using micro-injection and its role in the host-locating behavior of S. furcifera was investigated in an H-tube experiment. Real-time qPCR was used to determine the relative expression levels of OBP genes after RNAi treatment. [Results] The relative expression of SfOBP11 decreased significantly 24 h after RNAi treatment but mortality remained the same. RNAi treatment significantly reduced the ability of nymphs to find host plants. [Conclusion] SfOBP11 could play an important role in the detection of rice volatiles by S. furcifera.