【目的】 筛选和鉴定茶树中与假眼小绿叶蝉Empoasca vitis Göthe相关的抗性成分。【方法】 采用氨基酸分析仪和超高效液相色谱法分别分析8个茶树品种的氨基酸及其组分、咖啡碱和茶多酚及其组分的含量。采用人工饲料喂饲法对可能的抗性物质进行初步鉴定。【结果】 通过对假眼小绿叶蝉抗性水平差异较大的茶树品种的30多种生化物质的分析，筛选出抗、感茶树品种间差异较大的物质，包括茶多酚、天冬氨酸、γ-氨基丁酸、绿原酸和茶氨酸。分别将其作为可变因子设置不同浓度梯度，加入到人工饲料饲养假眼小绿叶蝉，统计5种化学成份不同浓度人工饲料上假眼小绿叶蝉的成活率，初步确定了这5种化学成份对假眼小绿叶蝉成活率的影响。其中，γ-氨基丁酸可能是茶树抗虫物质之一。【结论】 为阐明茶树抗叶蝉的化学机理奠定基础。

 [Objectives]  To identify chemicals in tea plant cultivars that contribute to resistance to Empoasca vitis Göthe. [Methods]  An amino acid analyzer and ultra performance liquid chromatography (UPLC) were used to analyze amino acids, caffeine and tea polyphenols obtained from different tea plant cultivars. By feeding E. vitis artificial diets that included different volatile compounds, we conducted a preliminarily identification of the chemicals associated with the resistance of different varieties of tea plants to this pest. [Results]  More than 30 biochemical compounds from 8 different tea varieties with different levels of resistance to E. vitis were analyzed. These compounds differed significantly in composition, particularly in the amount of polyphenols, aspartic acid , γ- aminobutyrate acid, chlorogenic acid, and theanine, they contained. Different concentrations of the above chemicals were added to artificial diets prepared for E. vitis in order to determine their effect on survival rates. The results of these feeding trials indicate that γ- aminobutyric acid may contribute to the resistance of tea plants to E. vitis. [Conclusion]  Certain volatile chemicals from tea plants may be important to their resistance to E. vitis.