槐绿虎天牛对柠条锦鸡儿6种挥发物的EAG和行为反应
EAG and behavioral responses of Chlorophorus diadema to sixvolatiles from Caragana kroshinskii
陈艳萍** 刘 强*** 李 敏 王星霁 张玲玲 曹丽娟 郄博蕊
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DOI：10.7679/j.issn.2095-1353.2016.080
作者单位：天津市动植物抗性重点实验室, 天津师范大学生命科学学院, 天津 300387
中文关键词：槐绿虎天牛，柠条锦鸡儿，挥发物，EAG，行为反应
英文关键词：Chlorophorus diadema, Caragana kroshinskii, volatiles, EAG, behavioral response
中文摘要：
【目的】 为了研究槐绿虎天牛Chlorophorus diadema（Motschulsky）对主要寄主植物柠条锦鸡儿Caragana kroshinskii（Kom.）挥发物中醇类、醛类、烷类、酯类和萜烯类共5类6种化合物的生物活性。【方法】 采用EAG和嗅觉行为生测技术，测定槐绿虎天牛雌雄虫对1-癸醇、正壬醛、正十六烷、乙酸-2-乙基丁酯、1-十六烯和1-辛烯6种化合物单一组分8个浓度梯度及其20种组合配方的电生理和嗅觉行为反应。【结果】 结果表明，槐绿虎天牛雌雄虫对单一化合物和组合配方的EAG反应值与嗅觉行为反应选择率的变化趋势基本一致。组合配方中，雌雄虫均对S、P、Q、H、I和B 6种配方的引诱活性与对照的差异性具有统计意义（P<0.05）。【结论】 （1）组合配方明显较单一组分配方对槐绿虎天牛的引诱效果好；（2）由1-癸醇、正十六烷和正壬醛组成的S配方引诱效果最好；（3）1-癸醇、正十六烷、正壬醛和1-十六烯4种化合物的最适浓度可以作为槐绿虎天牛植物源引诱剂的成分作进一步的研究。
英文摘要：
[Objectives] To investigate the bioactivity of six volatile alcohols, aldehydes, alkanes, esters and terpenes, from the plant Caragana kroshinskii (Kom.) to adult Chlorophorus diadema (Motschulsky). [Methods] Electroantennogram (EAG) and behavioral responses of adult C. diadema to eight different concentrations of 6 single chemicals (1-decanol, nonanal, hexadecane, 2-ethylbutyl acetate, 1-hexadecene and 1-octene), and 20 blends of these chemicals, were tested using an EAG and Y-tube olfactometer. [Results] Both EAG and olfactory behavioral responses of C. diadema adults to single volatile components and blends were basically the same. Six of the 20 blends, S, P, Q, H, I and B, were significantly attractive to female and male beetles (P<0.05). [Conclusion] (1) Blended volatiles are more attractive than single compounds; (2) Compound S, which was comprised of 1-decanol, hexadecane and nonanal, was the most attractive substance tested; (3) A mixture of four components (1-decanol, hexadecane, nonanal and 1-hexadecene) can serve as a basic of attractant for C. diadema in further studies.