【目的】 本文探究了大豆食心虫Leguminirora glycinioorella (Mats.) Obraztsov进入滞育以后体内糖类、脂质和水分含量的变化，以及不同寄主植物和滞育诱导期温度对大豆食心虫体内糖类、脂质和水分含量的影响。【方法】 通过蒽酮硫酸法和氯仿甲醇分离法测定了滞育前后、以大豆Glycine max (L.) Merr和野大豆Glycine soja Sieb. et Zucc.两种植物为寄主以及不同滞育诱导温度下的大豆食心虫体内脂质、水分、总糖、糖原和海藻糖的含量，结果利用SPSS17.0数据分析软件进行分析。【结果】 进入滞育阶段的大豆食心虫体内脂质、总糖、海藻糖和糖原含量显著增加，含水量显著下降。以大豆为寄主的大豆食心虫在滞育阶段体内水分含量、总糖和糖原含量较野大豆寄主的高，海藻糖含量较野大豆的低，差异均显著。不同滞育诱导温度下的大豆食心虫体内生化物质含量基本一致。【结论】 滞育前后大豆食心虫体内各种生化物质变化明显，以栽培大豆为寄主和以野大豆为寄主的大豆食心虫体内生化物质含量不同，而滞育诱导温度对大豆食心虫体内生化物质含量影响不大。

[Objectives]  To explore changes in the content of specific biochemical substances in the soybean pod borer after diapause, as well as the impact of different host plants and diapause-inducing temperatures on these substances. [Methods]  The anthrone-sulfuric acid and methanol-chloroform extraction methods were used to determine the lipid, water, total sugar, trehalose, and glycerol, content of soybean pod borers on different host plants at different diapause-inducting temperatures before, and during, diapause. The results were analyzed with SPSS17.0 data analysis software. [Results]  The lipid, total sugar, trehalose, and glycogen, content of soybean pod borers increased significantly when these entered diapause, but their water content decreased significantly. Soybean pod borers fed on cultivated soybeans had significantly higher water, total sugar, and glycerol, content, and significantly lower trehalose content, than those fed on wild soybeans. There were no significant differences in these biochemical substances between soybean pod borers kept under different diapause-inducting temperatures. [Conclusion]  The levels of different biochemical substances in the soybean pod borer were affected by host plant but not by temperature. Levels of the biochemical substances examined significantly differed before and during diapause.