中华真地鳖断触角再生的研究
Antenna regeneration in Eupolyphaga sinensis (Blattaria: Corydiidae)
谭梁飞1, 2** 朱 芬1 王小云1 高勇勇1 姚 璇2 张令要2 雷朝亮1**
点击：1346次 下载：7次
DOI：10.7679/j.issn.2095-1353.2016.136
作者单位：1. 华中农业大学昆虫资源研究所，武汉 430070；2. 湖北省疾病预防控制中心，武汉 430079
中文关键词：中华真地鳖，断触角，再生，分生增节，再生临界期
英文关键词：Eupolyphaga sinensis, antenna amputation, regeneration, segment increase, regenerative critical period
中文摘要：
【目的】 研究中华真地鳖Eupolyphaga sinensis (Walker) 断触角再生特征及再生临界期。【方法】 选取1～10龄健康的初龄若虫一条触角除基部3节外剪除，蜕皮后观察并统计触角的节数。选取生长一致健康的初龄4龄若虫，于第0～19日龄进行断触角处理，蜕皮后观察触角的再生情况和触角的节数。【结果】 不同虫龄的初龄若虫断去触角蜕皮后，均能通过再生使触角的节数增加。1～10龄的若虫一条触角除基部3节外剪除，蜕皮后下一龄的触角节数分别为5、8、12、13～15、15～17、16～19、18～20、13～15、20～23、22～25、24～28节。4龄的初龄若虫一条触角分别除基部0～2、3、5、10、15、20节外剪除，蜕皮后触角的节数分别为11～12、13～15、18～21、19～25、24～27节，其中一条触角除基部20节外剪除蜕皮后基本恢复正常节数。不同日龄的4龄若虫一条触角除基部3节外剪除蜕皮后，0～8日龄的处理若虫蜕皮后触角节数为13～15节，而12日龄及以后的处理若虫蜕皮后触角节数为5～6节。4龄若虫的断触角再生临界期为9～11日龄。【结论】 中华真地鳖在再生临界期内断触角后能够再生触角。
英文摘要：
[Objectives] This study was aimed to determine the characteristics and critical period of the regenerative antennae in Eupolyphaga sinensis (Walker). [Methods] Healthy early 1-10th instar nymphs were amputated one of their antennae and the number of antenna segments were documented after their molting. Then 0-19 day old early 4th instar nymphs were treated, observed and counted accordingly. [Results] Different instar age of nymphs of E. sinensis were able to increase the segments of their amputated antenna by regeneration. When antennal segments of 1-10th instar nymphs were amputated to only keep three basal segments, the number of antennal segments of the next instar after the nymph molted were restored as follow: 5, 8, 12, 13-15, 15-17, 16-19, 18-20, 20-23, 22-25, 24-28, respectively. When antennal segments of the 4th instar nymphs were amputated to keep 0-2,3,5,10,15 or 20 segments, the number of antennal segments of the next instar after the nymph molted were restored as follow: 11-12,13-15,15-19,18-21, 19-25 or 24-27, respectively. Among them, the antennae which were kept 20 segments basically restored to the normal number of segments after molting. Antennae of old nymphs in 4th instars were amputated to keep 3 segments during different day old: for the 0 to 8 days-old ones, the antenna segments were 13-15 after molting, and for the older than the12 days-old ones, the antenna segments were 5-6 after molting. Therefore, the critical period for regeneration antenna of 4th instar nymphs was from the 9-11 day-old. [Conclusion] The amputated antennae could regenerate within the regenerative critical period of E. sinensis.