韭菜迟眼蕈蚊对新烟碱类杀虫剂的抗药性及抗性机制初探
Neonicotinoid insecticide resistance in Bradysia odoriphaga Yang et Zhang
陈澄宇** 王翠翠 郭静静 许国升 史雪岩*** 宋敦伦
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DOI：10.7679/j.issn.2095-1353.2016.155
作者单位：中国农业大学植物保护学院，北京 100193
中文关键词：韭菜迟眼蕈蚊，新烟碱类杀虫剂，抗性监测，抗性机制
英文关键词：Bradysia odoriphaga, neonicotinoid insecticides, resistance monitoring, resistance mechanism
中文摘要：
【目的】 为明确韭菜迟眼蕈蚊Bradysia odoriphaga对新烟碱类杀虫剂的抗性水平及其抗性机制。【方法】 通过测定不同地区韭菜迟眼蕈蚊对3种新烟碱类杀虫剂吡虫啉、噻虫嗪和噻虫胺的敏感度，及通过增效剂实验和酶活性测定，初步探索抗性产生机制，为韭菜迟眼蕈蚊抗性治理提供依据。【结果与结论】 4个不同的韭菜迟眼蕈蚊田间种群对3种新烟碱类杀虫剂均产生了不同水平的抗性。其中，唐山种群对3种新烟碱杀虫剂均产生了较高的抗性。研究发现，唐山种群的7-乙氧基香豆素-O-脱乙基酶（ECOD）比活力为（3.89±0.31）pmol/（mg·pro·min），显著高于敏感品系。增效剂PBO对唐山种群的吡虫啉毒力的增效比为2.64，高于对敏感品系的增效比1.08。因此，P450s酶活性的升高与韭菜迟眼蕈蚊对吡虫啉的抗性有关。
英文摘要：[Objectives] To clarify the level of resistance of Bradysia odoriphaga to neonicotinoid insecticides and the mechanism responsible for this resistance. [Methods] Larvae of B.odoriphaga were collected from different regions of China and their sensitivity to three neonicotinoids; imidacloprid, thiamethoxam and clothianidin, examined. In addition, the P450s-related resistance mechanism to neonicotinoids was explored. [Results and Conclusion] Four field populations of B. odoriphaga had different resistance levels to the three neonicotinoids tested. Among these populations, the TS population had the highest resistance to all three neonicotinoids. ECOD activity of the TS population was (3.89±0.31)pmol/(mg·pro·min), higher than that of the SS population. Moreover, the synergic ratio of PBO to imidacloprid toxicity in the TS population was 2.64, which was higher than that of the susceptible population to PBO (1.08). This suggests that elevated P450s activity is related to imidacloprid resistance in B. odoriphaga larvae.