基于DNA条形码的物种界定算法比较研究
The reliability of DNA barcoding as a means of identifying Notodontid moth species (Insecta: Lepidoptera, Notodontidae) in Beijing, China in Beijing, Chinaptera, Notodontidae)
金 倩1, 2** 武春生3 陈 芬1 罗桂杰1 蔡卫佳1 刘 旭1 杨采青2
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DOI：10.7679/j.issn.2095-1353.2017.002
作者单位：1. 江苏省农业科学院宿迁农科所，宿迁 223800；2. 首都师范大学生命科学学院，北京 100048；3. 中国科学院动物研究所动物进化与系统学重点实验室，北京 100101
中文关键词：舟蛾科，DNA条形码，物种界定，GMYC，ABGD，jMOTU
英文关键词： Notodontidae, DNA barcoding, species delineation, GMYC, ABGD, jMOTU
中文摘要：
【目的】 为了探究3种常用物种界定方法（jMOTU、ABGD、GMYC）的界定效果。【方法】本研究以中国北京周边地区10个采样点483个舟蛾科样品为例，利用线粒体细胞色素C 氧化酶Ⅰ亚基基因（Cytochrome c oxidase subunitⅠ gene，COⅠ或COX1，约650 bp）的部分序列，进行3种物种界定算法（jMOTU、ABGD、GMYC）的实例比较研究。【结果】3种物种界定方法的鉴定效力存在差异，与形态学结果相比较，ABGD方法划分物种的准确率为100%，基于BEAST的GMYC模型结果与形态学结果一致，产生的置信区间（64~68）覆盖了形态学的结果（67）。然而，基于d8tree/MPLtree的GMYC方法倾向于高估MOTUs，jMOTU方法倾向于低估物种数目。【结论】 结果显示，ABGD方法和基于BEAST的GMYC模型方法对于本文研究对象舟蛾科能够较好地划分，可以对基于形态学的物种界定进行有效补充。
英文摘要：
[Objectives] To evaluate the performance of three species identification algorithms (jMOTU, ABGD, GMYC) with respect to the Notodontidae (Insecta, Lepidoptera). [Methods] COⅠ genes from 483 notodontid moth specimens collected from 10 collection sites in Northern China around Beijing were amplified using universal barcoding primers. [Results] Results obtained using each of the three algorithims differed. Using the results obtained by morphological species identification methods as a reference, the ABGD algorithm was 100% accurate. The results obtained using GMYC_BEAST were also generally consistent with those obtained by morphological methods, having a confidence interval of 64-68 c.f. 67 species identified by morphological methods. However, the GMYC_d8tree/MPLtree method tended to overestimate MOTUs and the jMOTU method tended to underestimate the number of species. [Conclusion] ABGD and GMYC_BEAST can reliably identify species within Notodontidae on the basis of COⅠ gene variation.