目的】 探明烟草对烟蚜Myzus persicae (Sulzer) 的抗性机制，为烟草抗性品种的选育及烟蚜的综合防治提供理论依据。【方法】 采用新叶子圆片法测定烟蚜在20个烟草品种上的发育历期、繁殖力、存活率等，并测定了20个烟草品种鲜叶可溶性蛋白质含量、pH值、叶绿素含量及叶背茸毛数量。【结果】 在不同烟草品种上烟蚜的选择性、成虫寿命、繁殖力、若蚜历期及存活率差异显著。其中闽烟38、贵烟3号和云烟97更感虫，而闽烟57、闽烟7号和中烟102具有较强的抗虫性。不同烟草品种鲜叶片中pH值的差异并不显著，但叶背茸毛密度、可溶性蛋白质含量、叶绿素a和叶绿体色素含量差异显著，且与烟蚜成虫量呈极显著正相关，这表明烟蚜更喜食叶背茸毛密度高、可溶性蛋白质含量高、叶绿素含量高的烟草品种。【结论】 20个烟草品种中，闽烟57、闽烟7号和中烟102具有较强的抗虫性。选育背茸毛密度低、可溶性蛋白质含量低、叶绿素含量低的品种，可提高烟草对烟蚜的抗性。

 [Objectives]  To explore the mechanisms underlying the resistance of tobacco to Myzus persicae (Sulzer), provide a theoretical basis for breeding resistant tobacco varieties and developing comprehensive prevention and control methods for M. persicae. [Methods]  We quantified the developmental duration, fecundity and survival rates of M. persicae on 20 tobacco varieties with a new leaf-disc assay, and measured the soluble protein, pH, chlorophyll content and leaf trichome density of fresh leaves of each variety. [Results]  Adult longevity, fecundity, duration of the nymph period, and survival, were significantly different on different tobacco varieties. Among the varieties examined, Minyan38, Guiyan3 and Yunyan97 had weak resistance, but Minyan57, Minyan7 and Zhongyan102were highly resistant. There was no obvious difference in the pH of fresh leaves of the different varieties, but there were highly significant, positive, correlations between trichome density, soluble protein content, chlorophyll a and chlorophyll pigment content of each tobacco variety and the number of M. persicae adults found on plants of each variety. M. persicae preferred varieties with high trichome density, and high soluble protein and chlorophyll content. [Conclusion]  Of the 20 tobacco varieties tested, Minyan57, Minyan7 and Zhongyan102 were the most highly resistant to M. persicae. Tobacco varieties with low trichome density, and low soluble protein and chlorophyll content, were more resistant to M. persicae.