黄胸散白蚁对马缨丹叶片提取物 的嗅觉行为反应
Behavioral responses of Reticulitermes flaviceps to Lantana camara leaf extracts
孙骊珠** 徐伟丽 罗 兰 袁忠林***
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DOI：10.7679/j.issn.2095-1353.2017.033
作者单位：青岛农业大学农学与植物保护学院，山东省植物病虫害综合防控重点实验室，青岛 266109
中文关键词： 黄胸散白蚁，马缨丹，“Y”型嗅觉仪，小滤纸片法，行为反应
英文关键词： Reticulitermes flaviceps, Lantana camara, Y-tube olfactometer, small filter paper disk, behavioral response
中文摘要：
【目的】 为了明确黄胸散白蚁Reticulitermes flaviceps对马缨丹Lantana camara叶片不同溶剂提取物的嗅觉行为反应。【方法】 利用“Y”型嗅觉仪测定了黄胸散白蚁饥饿与否、气流速度、观察时间对其嗅觉行为反应的影响，优化了测定条件；用小滤纸片法和优化条件下“Y”型嗅觉仪测定了马缨丹叶片不同溶剂提（萃）取物对黄胸散白蚁的嗅觉行为反应。【结果】 白蚁饥饿12 h，气流速度为2 L/min，10 min后观察为用“Y”型嗅觉仪测定白蚁对提（萃）取物嗅觉行为反应的最优条件。两种测定方法均表明，马缨丹干叶片氯仿提取物、乙醇提取物的乙酸乙酯和石油醚萃取物对黄胸散白蚁均具有驱避作用，随着提（萃）取物浓度的增大驱避作用越强。【结论】 马缨丹叶片中含对白蚁具有驱避作用的物质，为白蚁防治提供了新的途径，也为进一步研究提供了科学依据。
英文摘要：
[Objectives] To determine the olfactory behavioral responses of Reticulitermes flaviceps to Lantana camara leaf extracts. [Methods] Behavioral responses of fasted, and fed, R. flaviceps to chloroform, ethyl acetate and petroleum ether, extracts of L. camara leaf volatiles were measured in a Y-tube olfactometer under different airflow velocities and observation periods of different durations, and also by the small filter paper disk method. [Results] Optimal conditions for Y-tube olfactometer tests were when termites had been fasted for 12 h, the airflow velocity was 2 L/min, and the observation period was 10 min. The results of both the Y-tube olfactometer and small filter paper disk methods showed that the chloroform, ethyl acetate and petroleum ether, extracts of L. camara leaf volatiles were all repellent to termites; the higher the concentration of the extracts, the stronger the repellence. [Conclusions] L. camara leaves contain volatile substances that are repellent to R. flaviceps.