【目的】 明确黄粉虫幼虫Tenebrio molitor 体重增加和食物转化率提高的最适温度条件及肠道消化酶的种类及活性。【方法】 在实验室不同温度条件下，测定了不同日龄黄粉虫幼虫的体重、食物的近似消化率与转化率及肠道蛋白酶、淀粉酶和纤维素酶的活性。【结果】 温度对黄粉虫幼虫体重增加及食物的近似消化率（AD）和转化率（ECD）影响很大。在24~28℃时，3种不同日龄的黄粉虫幼虫体重明显高于20℃和32℃时的体重，28℃时60日龄和90日龄黄粉虫幼虫平均每天的体重增加量是同期20℃的1.26倍和1.35倍，且该虫对食物的近似消化率和转化率也表现出相同的规律。此外，温度也影响着黄粉虫幼虫体内消化酶的活力，当温度为24~28℃时，其肠道蛋白酶、淀粉酶、内切β-1，4-葡聚糖苷酶（Cx）和β-葡萄糖苷酶的活力均高于20℃和32℃时的活力，且不同龄期的黄粉虫幼虫体内4种消化酶的活力不同，随虫龄的增加4种消化酶活力明显升高。【结论】 黄粉虫幼虫在24~28℃时对食物的转化率最高，体重增加最快，高龄黄粉虫幼虫肠道内4种消化酶活力均明显高于低龄幼虫。

 [Objectives]  To explore optimum temperature on weight gain, food conversion efficiency and digestive enzyme activities of Tenebrio molitor larvae. [Methods]  The rate of approximate digestibility, body weight, conversion efficiency, and digestive enzyme activities of different larval instars of T. molitor were investigated under different temperatures. [Results]  The results showed that temperature had great influence on weight gain, approximate digestibility, and conversion efficiency. The weight of larval raised at 24℃ and 28℃ were significantly higher than that of raised at 20℃ and 32℃. At 28℃, the average daily weight gain of 60-day-old and 90-day-old larvae were 1.26 and 1.35 times greater than those 20℃, respectively. The approximate digestibility and conversion efficiency showed similar patterns with changing temperature conditions. In addition, temperature influenced the digestive enzyme activities of the larvae. The activity of intestinal protease, amylase, endo β-1, 4-β-glucan glycosidase (Cx), and β-glucosidase at 24-28℃ were all higher than 20℃ or 32℃. The activities of these digestive enzymes also varied with larval age. [Conclusion]  The optimal temperature of conversion efficiency and weight gain of T. molitor larvae were 24℃ and 28℃. The activities of 4 digestive enzymes statstically increases as the larvae grew older.