双斑长跗萤叶甲对棉花和玉米七种 挥发物的EAG及行为反应
Electrophysiological and behavioral responses of Monolepta hieroglyphica (Motschulsky) to 7 cotton and corn volatiles
郭丹丹** 张志虎 陈 静*** 王少山***
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DOI：10.7679/j.issn.2095-1353.2018.013
作者单位：农业部西北荒漠绿洲作物有害生物综合治理重点实验室，新疆绿洲农业病虫害治理与植保资源利用 自治区普通高校重点实验室，石河子大学农学院，石河子 832003
中文关键词：双斑长跗萤叶甲，棉花，玉米，挥发物，电生理反应，行为反应
英文关键词： Monolepta hieroglyphica, cotton, corn, volatiles, EAG responses, behavioral response
中文摘要：
【目的】 为筛选适合配制双斑长跗萤叶甲Monolepta hieroglyphica（Motschulsky）引诱剂的有效成分。【方法】 本试验采用昆虫触角电位反应仪和“Y”型嗅觉仪测定了双斑长跗萤叶甲雌、雄成虫对棉花和玉米7种挥发物的电生理反应和行为反应。【结果】 双斑长跗萤叶甲雌、雄成虫均对10 μg·mL-1和1 μg·mL-1的γ-萜品烯、10 μg·mL-1的D-柠檬烯、10 μg·mL-1的β-紫罗酮的EAG反应较明显，对10 μg·mL-1和10-2 μg·mL-1的α-石竹烯和10 μg·mL-1、1 μg·mL-1和10-2 μg·mL-1的氧化石竹烯的反应次之，对十二烷、十三烷的反应较不明显。t-检验表明，雌、雄成虫对挥发性物部分浓度的EAG反应存在显著差异。行为反应测定结果表明，在10 μg·mL-1刺激剂量条件下，β-紫罗酮对雌虫表现出明显的引诱作用，γ-萜品烯和氧化石竹烯对其有显著的驱避作用；γ-萜品烯和D-柠檬烯对雄虫具有显著的引诱作用，β-紫罗酮对其具有显著的驱避作用。【结论】 该研究结果可为双斑长跗萤叶甲田间引诱剂的研发提供借鉴。
英文摘要：
[Objectives] To identify chemical lures for trappin maleand femalMonolepthieroglyphica (Motschulsky). [Methods] Electroantennography and a Y-shaped olfactometer were used to test the electroantennography (EAG) and behavioral responses of M. hieroglyphica to selected cotton and corn volatiles. [Results] The results show that the most significant EAG responses of M. hieroglyphica males and females were elicited by 10 μg·mL-1 and 1 μg·mL-1 of γ-terpinene, 10 μg·mL-1 of D-limonene, and 10 μg·mL-1 of β-Ionone, followed by 10 μg·mL-1 and 10-2 μg·mL-1 of α-caryophyllene, and 10 μg·mL-1, 1 μg·mL-1 and 10-2 μg·mL-1 of caryophyllene oxide. Both males and females had a lower response to odecane and tridecane. A Student’s t-test showed significant differences in the EAG responses of males and females to partial concentrations of volatiles. The results of Y-shaped olfactometer bioassays indicate that females were attracted by 10 μg/mL of β-Ionone, but were repelled by γ-terpinene and caryophyllene oxide. Males were attracted by γterpinene andDlimonene but repulsed by β-Ionone. [Conclusion] These results can provide a basis for developing attractants for M. hieroglyphica in the field.