家蚕幼虫咽侧体体积变化分析
Change in the corpus allatum of silkworm, Bombyx mori, larvae
李亚腾；张 亮；李 田；王小康；赵 威； 陈 萍
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DOI：10.7679/j.issn.2095-1353.2018.087
作者单位： 西南大学生物技术学院，重庆 400715；农业部蚕桑生物学与遗传育种重点实验室，重庆 400715
中文关键词： 家蚕，咽侧体，腺细胞，体积
英文关键词： silkworm, corpus allatum, glandular cells, volume
中文摘要：
【目的】 本试验旨在研究不同生长发育时期家蚕幼虫咽侧体及其腺细胞的变化。【方法】 选取家蚕4眠蚕中限品种作为实验材料，对其4龄、5龄等时期进行解剖处理，得到咽侧体后进行透析处理与DAPI染色，在荧光显微镜下成荧光像图，结合软件分析工具测量咽侧体横截面积直径与细胞数量来确定家蚕咽侧体及其腺细胞在不同生长时期的变化。【结果】 咽侧体体积随幼虫生长发生了明显增长；咽侧体细胞数目随幼虫生长会增加，但比较缓慢。随着幼虫的生长，腺细胞体积会增大，同时咽侧体体积增长与腺细胞核体积增长有极显著相关性。【结论】 家蚕咽侧体体积增长主要源于腺细胞体积的增大而稍带腺细胞数目的增加，家蚕幼虫咽侧体体积与生长发育的时间经过密切相关。
英文摘要：
[Objectives] To investigate changes in the corpus allatum
and glandular cells of silkworm larvae at different stages of development. [Methods] The corpus allatum was dissected from
fourth and and fifth instar larvae of the tetramolter Zhongxian variety of
silkworm. Fluorescent images of the corpus allatum and glandular cells were
obtained using a fluorescent microscope after dialysis treatment and DAPI
staining and combined with an analysis tool to measure the cross sectional area
of the corpus allatum and the number of glandular cells. [Results] The volume of the
corpus allatum increased significantly as larvae developed. The number of
glandular cells also increased with the development but this was relatively
slow. As larvae developed, the volume of glandular cells increased, and the
volume of the corpus allatum was significantly correlated with glandular cell
size. [Conclusion] The increase in the volume of the corpus
allatum in silkworm larvae is mainly due to the increased volume of glandular
cells. The number of glandular cells and the volume of the corpus allatum is
closely related to the developmental stage of larvae.