刊期:双月刊
主管单位:中国科学院
主办单位:中国科学院动物研究所,中国昆虫学会
地址:北京市朝阳区北辰西路1号院5号中国科学院动物研究所
邮编:100101
电话:010-64807137
传真:010-64807137
E-Mail:entom@ioz.ac.cn
刊号:ISSN 2095-1353
        CN 11-6020/Q
国内发行代号:2-151
国际发行代号:BM-407
发行范围:国内外公开发布
定价:138元/册
定价:828元/年
银行汇款:中国工商银行北京海淀西区支行
户名:中国科学院动物研究所
帐号:0200 0045 0908 8125 063

您所在位置:首页->过刊浏览->2019年56卷第1期



基于细胞色素C氧化酶亚基I基因的 小蜡螟系统进化分析
Phylogenetic analysis of Achroia grisella Fabricius based on the mitochondrial cytochrome c oxidase subunit I gene
张永红** 朱 峰 唐芬芬 邵榆岚 杨 爽 白兴荣***
点击:1804次 下载:8次
DOI:10.7679/j.issn.2095-1353.2019.013
作者单位:云南省农业科学院蚕桑蜜蜂研究所,蒙自 661101
中文关键词:小蜡螟;CO I基因;同源性;进化分析
英文关键词: Achroia Grisella Fabricius; CO I gene; homoeology; evolution analysis
中文摘要:目的】 小蜡螟Achroia Grisella Fabricius作为蜂业生产中的主要虫害,为确认它在鳞翅目昆虫中的遗传地位,本研究克隆了小蜡螟细胞色素C氧化酶亚基I基因CO I,并对其序列进行相关生物信息学分析。【方法】 根据NCBI已登录鳞翅目昆虫线粒体基因组mtDNA 中CO I基因保守序列设计简并引物,以小蜡螟基因组为模板进行PCR,克隆并测序得到小蜡螟mtDNA CO I基因序列(GenBank登录号:MF509586)。【结果】 CO I基因编码区进行序列分析发现,其翻译的起始码子为TTG,以碱基T作为不完全终止;通过CO I基因同源性和遗传距离分析发现,小蜡螟与大蜡螟Galleria mellonella同源性最高且遗传距离最近,分别为88.3%、0.117。系统进化分析发现,小蜡螟与其它鳞翅目昆虫聚为一支,其中螟蛾科昆虫大蜡螟、小蜡螟、米蛾Corcyra cephalonica和梢斑螟Corcyra cephalonica聚在一个小的分支。【结论】 小蜡螟与大蜡螟的进化地位最为相近,也说明了利用CO I基因可以区分同属不同种的蜡螟,研究结果为了解小蜡螟的遗传背景提供理论基础。
英文摘要: [Objectives]  Achroia grisella Fabricius (small wax moth) is the main pests in the apiculture industry, in order to confirm its position of the genetic in Lepidoptera insects, cytochrome C oxidase subunit I gene (CO I) of small wax moth was cloned, and analyzed by bioinformatics analysis. [Methods]  According to the conserved sequence of mitochondrial genome mtDNA CO I gene was logged in lepidoptera insects in NCBI, the degenerate primers were designed for the study, the genome of small wax moth as a template for PCR, mtDNA CO I sequence (GenBank accession NO. MF509586) of small wax moth was cloned and sequenced. [Results]  The sequence analysis of the CO I gene coding region found that the initial code of its translation was TTG, and the base T was not terminated completely. The homology and genetic distance analysis of CO I genes showed that Galleria mellonella (greater wax moth) and small wax moth were the most homologous and the genetic distance was 88.3% and 0.117 respectively. Phylogenetic analysis found that the small wax moth and other lepidoptera insects formed a separate clade, this clade gathered in a small branch that include greater wax moth, small wax moth, the rice moth (Corcyra cephalonica) and Corcyra cephalonica. [Conclusion]  A. grisella was most closely related to G. mellonella, CO I gene was used for distinguishing the different kinds of wax moths from same genus, the research of results provided a theoretical basis for understanding the genetic background of small wax moth.
读者评论

      读者ID: 密码:   
我要评论:
版权所有©2024应用昆虫学报》编辑部 京ICP备10006425号
本系统由北京菲斯特诺科技有限公司设计开发
您是本站第8900370名访问者