基于细胞色素C氧化酶亚基I基因的 小蜡螟系统进化分析
Phylogenetic analysis of Achroia grisella Fabricius based on the mitochondrial cytochrome c oxidase subunit I gene
张永红** 朱 峰 唐芬芬 邵榆岚 杨 爽 白兴荣***
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DOI：10.7679/j.issn.2095-1353.2019.013
作者单位：云南省农业科学院蚕桑蜜蜂研究所，蒙自 661101
中文关键词：小蜡螟；CO I基因；同源性；进化分析
英文关键词： Achroia Grisella Fabricius; CO I gene; homoeology; evolution analysis
中文摘要：目的】 小蜡螟Achroia Grisella Fabricius作为蜂业生产中的主要虫害，为确认它在鳞翅目昆虫中的遗传地位，本研究克隆了小蜡螟细胞色素C氧化酶亚基I基因CO I，并对其序列进行相关生物信息学分析。【方法】 根据NCBI已登录鳞翅目昆虫线粒体基因组mtDNA 中CO I基因保守序列设计简并引物，以小蜡螟基因组为模板进行PCR，克隆并测序得到小蜡螟mtDNA CO I基因序列（GenBank登录号：MF509586）。【结果】 对CO I基因编码区进行序列分析发现，其翻译的起始码子为TTG，以碱基T作为不完全终止；通过CO I基因同源性和遗传距离分析发现，小蜡螟与大蜡螟Galleria mellonella同源性最高且遗传距离最近，分别为88.3%、0.117。系统进化分析发现，小蜡螟与其它鳞翅目昆虫聚为一支，其中螟蛾科昆虫大蜡螟、小蜡螟、米蛾Corcyra cephalonica和梢斑螟Corcyra cephalonica聚在一个小的分支。【结论】 小蜡螟与大蜡螟的进化地位最为相近，也说明了利用CO I基因可以区分同属不同种的蜡螟，研究结果为了解小蜡螟的遗传背景提供理论基础。
英文摘要： [Objectives] Achroia grisella Fabricius (small wax
moth) is the main pests in the apiculture industry, in order to confirm its
position of the genetic in Lepidoptera insects, cytochrome C oxidase subunit I
gene (CO I) of small wax moth was
cloned, and analyzed by bioinformatics analysis. [Methods] According to the
conserved sequence of mitochondrial genome mtDNA CO I gene was logged in lepidoptera insects in NCBI, the degenerate
primers were designed for the study, the genome of small wax moth as a template
for PCR, mtDNA CO I sequence (GenBank
accession NO. MF509586) of small wax moth was cloned and sequenced. [Results] The sequence analysis of the CO I gene coding region found that the
initial code of its translation was TTG, and the base T was not terminated
completely. The homology and genetic distance analysis of CO I genes showed that Galleria
mellonella (greater wax moth) and small wax moth were the most homologous
and the genetic distance was 88.3% and 0.117 respectively. Phylogenetic
analysis found that the small wax moth and other lepidoptera insects formed a
separate clade, this clade gathered in a small branch that include greater wax
moth, small wax moth, the rice moth (Corcyra
cephalonica) and Corcyra cephalonica. [Conclusion] A.
grisella was most closely related to G.
mellonella, CO I gene was used
for distinguishing the different kinds of wax moths from same genus, the
research of results provided a theoretical basis for understanding the genetic
background of small wax moth.