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漂白粉对家蚕中肠产消化酶细菌的影响
Effects of bleach powder on the midgut digestive enzymes of silkworms
李 畅1** 周 沂1** 张玉琴1 陈 林1 杨仁奎2*** 冯丽春1***
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DOI:10.7679/j.issn.2095-1353.2019.018
作者单位:1. 农业农村部蚕桑生物学与遗传育种重点实验室,西南大学生物技术学院,重庆 400716; 2. 重庆市蚕业科学技术研究院,重庆 400700
中文关键词:漂白粉;家蚕;中肠;产酶菌
英文关键词: bleaching powder; silkworm; midgut; enzyme producing bacteria
中文摘要:

【目的】 本研究以漂白粉液浸渍消毒(以下简称浸消)桑叶饲喂家蚕为处理组,分析了不同组之间家蚕中肠细菌数量的差异;探讨了各组家蚕中肠产消化酶(蛋白酶、淀粉酶、脂肪酶、纤维素酶)细菌的菌株数量、产酶种类及相对产酶能力的差异。【方法】 用16S rDNA测序技术对产酶菌株进行了鉴定,比较各组产酶菌种类的不同。【结果】饲喂不同有效氯浓度漂白粉液浸消叶对家蚕中肠细菌的数量、产酶菌数量均有抑制作用,且随有效氯浓度升高抑制作用增强;各处理组产蛋白酶菌株平均相对产酶能力均低于清水组,且清水组肠液中产淀粉酶菌株数明显多于各处理组;取食漂白粉液浸消桑叶后,家蚕中肠产消化酶细菌类群构成有一定变化,清水组和全程0.3%组产酶菌属相对较丰富,有Staphylococcus sp.Lelliottia sp.Buttiauxella sp.,其他处理组的产酶菌属相对单一,只有Staphylococcus sp.【结论】 以上结果说明漂白粉对家蚕中肠内细菌及产酶菌的增殖有抑制作用,中肠内产蛋白酶菌的产酶能力和产淀粉酶菌株的数量也受到抑制,对产酶菌类群构成有一定影响。

英文摘要:

[Objectives]  To investigate the effect of bleach powder on silkworm intestinal bacteria and digestive enzymes, [Methods]  Silkworms were either fed mulberry leaves that had been disinfected with bleach powder or untreated mulberry leaves, and their intestinal bacteria were isolated and identified by 16S rDNA sequencing. [Results]  The overall amount of bacteria, and of enzyme-producing bacteria,in the midgut was generally inhibited by bleach powder, regardless of the effective chlorine concentration although there was a positive correlation between the strength of this inhibitory effect and chlorine concentration, Compared to the Control, the abundance of amylase-producing bacteria, as well as the average production of protease, were significantly lower in larvae that had been fed leaves treated with bleach powder, With the exception of the the lowest effective chlorine concentration, 0.3%, exposure to all other concentrations of bleach powder reduced the diversity of mid-gut bacteria to a single genus; Staphylococcus sp., In contrast, the control group and the 0.3% treatment group had relatively diverse enzyme-producing bacteria, including Staphylococcus sp., Lelliottia sp., and Buttiauxella sp. [Conclusion]  Both intestinal bacterial community diversity and bacterial digestive enzymes werre significantly inhibited by bleach powder, particularly bacterial protease production and the abundance of amylase-producing bacteria.


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