牧草盲蝽对七种寄主植物不同挥发物的EAG和行为反应
EAG and olfactory behavioral responses of Lygus pmtensis to volatiles from seven host plants species
孙 鹏** 迪丽努尔?艾麦提 苟长青 冯宏祖***
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DOI：10.7679/j.issn.2095-1353.2019.039
作者单位：塔里木大学，南疆特色果树高效优质栽培与深加工技术国家地方联合工程实验室，农业部阿拉尔作物有害生物科学观测实验站，塔里木大学南疆农业有害生物综合治理重点实验室，阿拉尔 843300
中文关键词：牧草盲蝽；寄主植物；挥发物；EAG反应；趋向行为
英文关键词：Lygus pmtensis; host plants; volatiles; EAG response; taxis behavior
中文摘要：【目的】 研究牧草盲蝽Lygus pmtensis对寄主植物挥发物的EAG及趋向行为反应，为研发牧草盲蝽引诱剂或趋避剂策略提供科学依据。【方法】 利用固相微萃取方法收集7种寄主植物包括木地肤Kochia prostrata (L.) Schrad.、灰绿藜Chenopodium glaucum、花椰菜Brassica oleracea L. var. botrytis L.、小白菜Brassica campestris L. ssp. chinensis Makino、田旋花Convolvulus arvensis L.、小藜Chenopodium serotinum L.、番茄Lycopersicon esculentum Mill.的挥发物，通过室内气相-质谱联用系统（GC-MS）进行挥发物的定性及定量分析，最终测定了9种候选特征挥发物的触角电位（EAG）反应和嗅觉趋向行为反应。【结果】 7种寄主植物共鉴定到的化合物有31种，且种类和含量有较大差异，差异显著。9种候选特征挥发物均能引起牧草盲蝽雌、雄成虫的EAG反应。其中牧草盲蝽雌、雄成虫对壬醛的EAG反应绝对值最大，雌、雄虫对不同植物挥发物质EAG反应相对值均有显著差异，且对同种化合物雌虫的反应相对值均大于雄虫的反应，乙酸顺式-3-己烯酯和壬醛引发的EAG反应值差异显著；趋向行为测定中，牧草盲蝽雌虫对壬醛、金合欢烯、α-蒎烯、1-石竹烯、乙酸顺式-3-己烯酯、2-甲基-1-醇的大部分浓度的行为反应差异显著，雄虫仅对α-蒎烯、壬醛的部分浓度的行为反应差异显著。【结论】 7种寄主植物的挥发物对牧草盲蝽雌、雄成虫具有不同EAG和趋向行为反应影响，候选气味化合物可以作为设计牧草盲蝽引诱剂组分进行田间害虫防控实践。
英文摘要： [Objectives] To identify potential attractants, or repellents, for use in the IPM of Lygus pmtensis. [Methods] The EAG and behavioral responses of L. pmtensis to volatiles from seven different host plants (Kochia prostrata (L.) Schrad., Chenopodium glaucum, Brassica oleracea L. var. botrytis L., Convolvulus arvensis L., Brassica campestris L. ssp. chinensis Makino, Chenopodium serotinum L., Lycopersicon esculentum Mill.) were measured and compared. Volatile organic compounds from the different host plants were sampled using SPEM and then identified and quantified with GC-MS. EAG and behavioral responses to 9 candidate volatiles from different host plants were then measured and compared. [Results] Thirty-one distinct volatile compounds were isolated from the seven host plants. There was a significant difference in volatiles between species. All 9 of the candidate volatiles tested elicited EAG responses; the strongest EAG response was elicited by Nonanal. There were significant differences in the EAG responses of males and females to the volatile compounds tested; females responded to 3-Hexen-1-ol, acetate, (Z)- and Nonanal more strongly than males. Females also had significantly different behavioral responses to Nonanal, 1,6,10-Dodecatriene, 7,11-dimethyl-3-methylene-, (E)-, α-Pinene, Caryophyllene, 3-Hexen-1-ol, acetate, (Z)-, 1-Hexanol, 2-ethyl. [Conclusion] Male and female L. pmtensis have different EAG and behavioral responses to volatiles from different host plants. Some potential attractants for the control of L. pmtensis were identified.