温度对柑橘大实蝇滞育蛹能量结构与代谢的影响
Effect of temperature on the energy metabolism and related enzyme activity of the Chinese citrus fruit fly, Bactrocera minax (Enderlein), during diapause
周志雄** 夏振洲 袁江江 王在凌 李传仁***
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DOI：10.7679/j.issn.2095-1353.2019.053
作者单位：（长江大学昆虫研究所，荆州 434025）
中文关键词：柑橘大实蝇；滞育；越冬温度；能量代谢
英文关键词：Bactrocera minax; diapause; overwintering temperature; energy metabolism
中文摘要：
【目的】 为从生理生化水平上探讨越冬温度对柑橘大实蝇Bactrocera minax（Enderlein）滞育蛹的调控生理机制。【方法】 测定了不同恒温条件下柑橘大实蝇越冬蛹滞育期虫体粗脂肪和糖类物质含量的变化动态。【结果】 低温（12 ℃）越冬促进了虫体粗脂肪的积累，随温度升高，粗脂肪含量逐渐减少；总糖含量随越冬温度升高，趋于倒“U”型变化；虫体海藻糖含量先升后降（24 ℃除外），随温度上升，海藻糖含量最高值时期逐渐提前，且最大值逐渐降低；虫体糖原含量变化趋势各不相同，随温度升高，糖原含量最高值时期逐渐接近滞育解除期，越冬温度升高促进了柑橘大实蝇越冬蛹滞育期后期糖原的积累。此外，16 ℃下越冬，同时期各物质的含量均低于其它处理，说明在16 ℃下越冬加快了虫体能量物质的代谢或转换。【结论】 不同恒温越冬，柑橘大实蝇越冬滞育期能量代谢方式不同，表明越冬温度与越冬蛹的能量代谢特点存在密切的关系。本研究结果对深入了解柑橘大实蝇越冬蛹能量代谢生理机制具有一定的参考价值。
英文摘要：
[Objectives] To understand the physiological and
biochemical mechanisms regulating the overwintering temperature of diapausing
pupae of the Chinese citrus fruit fly, Bactrocera
minax. [Methods] Crude fat, total carbohydrate, trehalose and
glycogen content of diapausing pupae at different overwintering temperatures
were measured and compared. [Results] A low overwintering temperature (12 ℃) promoted the accumulation of crude
fat, which gradually decreased with temperature. The trend in total
carbohydrate content tended to follow an inverted “U” shape with increasing
temperature. Similarly, the trend in trehalose content was of the “up-down” type
(except at 24 ℃).
Higher overwintering temperatures gradually advanced the peak of trehalose
content but also gradually decreased the maximum trehalose content of
diapausing pupae. The trend in glycogen content was different; higher
overwintering temperatures caused glycogen content to peak closer to the period
of diapause termination and also promoted the accumulation of glycogen in the
late diapause period. In addition, levels of all substances measured were
lowest at 16 ℃,
indicating that overwintering at this temperature accelerated the metabolism,
or conversion, of energy-related substances in pupae. [Conclusion] These results
suggest that different overwintering temperatures change the energy metabolism
of pupae, and that overwintering temperature is closely related to the energy
metabolism characteristics of diapausing pupa. The results of this study aid
understanding the physiological mechanisms of energy metabolism in the Chinese
citrus fruit fly.