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西花蓟马聚集信息素组分在两种载体中的释放规律及引诱效果
Comparison of two types of aggregation pheromone dispensers for trapping Frankliniella occidentalis (Thysanoptera: Thripidae)
孙冉冉;李晓维;章金明;张治军;张立猛;吕要斌
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DOI:10.7679/j.issn.2095-1353.2020.020
作者单位:南京农业大学植物保护学院,南京 210095;浙江省农业科学院植物保护与微生物研究所,农产品质量安全危害因子与风险防控国家重点实验室(筹),杭州 310021;云南省烟草公司玉溪市公司,玉溪 653100;烟草行业病虫害生物防治工程研究中心,玉溪 653100)
中文关键词:聚集信息素;橡胶塞;PVC管;释放模式;诱集效果
英文关键词:aggregation pheromone; rubber septa; PVC hollow fibers; release pattern; trap catches
中文摘要:
【目的】 应用蓟马聚集信息素是蓟马害虫绿色防控的重要措施。本研究旨在明确载体中西花蓟马聚集信息素2种组分(S)-2-甲基丁酸橙花酯和(R)-乙酸薰衣草酯的释放规律,为开发有效的诱芯提供指导。【方法】 应用固相微萃取(SPME)和气相色谱-质谱联用技术在室内测定2种载体中2种组分连续7 d的释放速率,并测定2种组分最佳配比时,2种组分的释放比值;通过田间诱集试验,比较2种载体诱芯的田间诱集效果。【结果】 结果表明,橡胶塞和PVC管2种载体中2种组分的释放规律相反。相同剂量下,橡胶塞中(S)-2-甲基丁酸橙花酯的释放速率高于PVC管中释放速率,而橡胶塞中(R)-乙酸薰衣草酯的释放速率低于PVC管中释放速率。当(S)-2-甲基丁酸橙花酯和(R)-乙酸薰衣草酯8︰1混合加入载体时,橡胶塞中2种组分2-7 d的释放比值在5.84-19.24之间,与西花蓟马聚集信息素组分的释放比值相似。而PVC管中2种组分2-7 d的释放比值相对稳定,维持在4左右。田间诱集试验结果表明,当中(S)-2-甲基丁酸橙花酯和(R)-乙酸薰衣草酯比例为8︰1时,橡胶塞载体诱芯的诱集效果优于PVC管载体诱芯。【结论】 橡胶塞是西花蓟马聚集信息素释放的合适载体。另外,由于PVC管中2种组分释放比值相对稳定,优化加样比例后其也可作为聚集信息素释放的候选载体。
英文摘要:
[Objectives]  To compare the performance of two types of pheromone dispensers for releasing two aggregation pheromones of Frankliniella occidentalis [neryl (S)-2-methylbutanoate and (R)-lavandulyl acetate] and thereby inform the choice of pheromones and pheromone dispensers for controlling these pests. [Methods]  The release profiles of the 2 active compounds from pheromone dispensers with a rubber septum or hollow PVC fibers were compared in a laboratory over a 7-day period using with solid phase microextraction (SPME) and a gas chromatography-mass spectrometer (GC-MS). In addition, the effect of time on the release ratios of N(S)2MB to (R)-LA was investigated to determine the optimal blend of these two compounds in both types of dispenser. The trapping efficacy of both types of dispenser baited with an optimal blend of aggregation pheromone compounds was then compared in field trials. [Results]  The laboratory experiments show that the amount of neryl (S)-2-methylbutanoate and (R)-lavandulyl acetate released from the two different types of dispenser were markedly different. Over the 7-day experimental period dispensers with a rubber septa released more neryl (S)-2- methylbutanoate, and less (R)-lavandulyl acetate, than those with hollow PVC fibers. In addition, dispensers with rubber septa loaded with a ratio of 8︰1 neryl (S)-2-methylbutanoate to (R)-lavandulyl acetate released these pheremones at a ratio ranged from 5.84 to 19.24, which is within the natural range of F. occidentalis. Dispensers with PVC hollow fibers had a relatively stable release ratio of about 4. Furthermore, field trials indicate that dispensers with rubber septa performed better than those with hollow PVC fibers with respect to trapping F. occidentalis adults. [Conclusion]  These results suggest that dispensers with rubber septa are superior to those with hollow PVC fibres for dispensing the aggregation pheromones of F. occidentalis. However, hollow PVC fibers can also perform well if the ratio of pheromones is optimized when the dispenser is loaded.
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