不同地理种群赤拟谷盗肠道细菌群落多样性分析
Diversity of intestinal bacteria in different geographic populations of Tribolium castaneum (Coleoptera: Tenebrionidae)
王争艳；王 洋；何梦婷；鲁玉杰
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DOI：10.7679/j.issn.2095-1353.2020.062
作者单位：河南工业大学，粮油食品学院；河南工业大学，粮油食品学院；河南工业大学，粮油食品学院；河南工业大学，粮油食品学院
中文关键词：赤拟谷盗；肠道细菌；多样性；16S rDNA；高通量测序
英文关键词：Tribolium castaneum; gut bacteria; diversity; 16S rDNA; high-throughput sequencing
中文摘要：
【目的】 了解储粮害虫赤拟谷盗Tribolium
castaneum (Herbst)成虫肠道细菌群落组成及多样性。【方法】 利用Illumina MiSeq高通量测序技术对3个地理种群的赤拟谷盗雄虫肠道细菌16S rDNA的V3-V4变异区进行测序分析。【结果】 从赤拟谷盗肠道样本中共测得465 293条reads，聚类为113个OTU，共注释到8门、15纲、33目、52科和79属的细菌。其中，优势菌门包括变形菌门（Proteobacteria）、厚壁菌门（Firmicutes）和放线菌门（Actinobacteria）等，优势菌属包括假单胞菌属（Pseudomonas）、肠杆菌科未鉴定属（Unclassified-f-Enterobacteriaceae）、不动杆菌属（Acinetobacter）、肠球菌属（Enterococcus）和根瘤菌属（Rhizobium）等。3个地理种群的赤拟谷盗肠道细菌的Shannon指数、Simpson指数、Ace指数和Chao指数分别为1.5-1.7、0.3、79.7-133.2和76.4-85.2。【结论】 不同地理种群的赤拟谷盗成虫的肠道细菌群落组成存在显著差异。
英文摘要：
[Objectives] To reveal the community structure and diversity of the intestinal bacteria of Tribolium castaneum. [Methods] The Illumina MiSeq high-throughput sequencing platform was used to sequence the V3-V4 regions of the bacterial 16S rDNA gene in the intestine of adult male T. castaneum from three geographic populations. [Results] A total of 465 293 reads and 113 OTUs were obtained and annotated into 8 phyla, 15 classes, 33 orders, 52 families and 79 genera. The most common phyla were the Proteobacteria, Firmicutes, and Actinobacteria. The most abundant genera were Pseudomonas, unclassified-f-Enterobacteriaceae, Acinetobacter, Enterococcus and Rhizobium. The Shannon, Simpson, Ace and Chao indices were 1.5-1.7, 0.30, 79.7-133.2 and 76.4-85.2, respectively. [Conclusion] Bacterial community structure and diversity differ among different geographic populations of T. castaneum.