【目的】 西花蓟马Frankliniella occidentalis (Pergande)是番茄斑萎病毒（Tomato spotted wilt orthotospovirus，TSWV）的主要传播媒介，以循环增殖方式传播TSWV，但在传播过程中参与的互作蛋白尚不清楚。本研究以TSWV的外鞘糖蛋白G_N（Glycoprotein N）为诱饵筛选与西花蓟马互作的蛋白，为阐明TSWV与西花蓟马的互作机制提供依据。【方法】 构建以pPR3-N为载体的西花蓟马泛素分裂酵母双杂交膜系统cDNA文库，利用Gene Ontology（GO）通路注释互作蛋白，分析其生物学功能。【结果】 西花蓟马cDNA文库库容为3.2×10⁶，除去重复序列、载体序列和移码序列，筛选到74个与G_N互作的蛋白, 参与了细胞过程、代谢过程、生物调节过程等12种生物过程。【结论】 成功构建了西花蓟马酵母双杂交膜体系cDNA文库，筛选到与TSWV G_N互作的蛋白，为进一步研究TSWV与西花蓟马的互作机制奠定了基础。

Objectives]  The  thrips Frankliniella occidentalis (Pergande) is the chief vector of the Tomato spotted wilt orthotospovirus (TSWV). This virus is transmitted in a cyclic and proliferative pattern, but the interactive proteins involved in this process are unclear. The G_N protein can be used as an indicator of interaction between F. occidentalis and TSWV, and  thereby provide a way to clarify the interactions between vector, virus and plant host. [Methods]  A cDNA library of the ubiquitin yeast, two-hybrid membrane system was constructed using the pPR3-N vector. Interacting proteins were annotated with the Gene Ontology (GO) pathway and their biological functions analyzed. [Results]  The capacity of the cDNA library is 3.2×10⁶. After the removal of repeat sequences, vector sequences and frameshift sequences, 74  thrips proteins were found to have interacted with TSWV G_N protein. Annotation results showed that these host factors were involved in 12 biological processes via the GO pathway, including cellular, metabolic and biological regulatory processes. [Conclusion]  A cDNA library of F. occidentalis was successfully constructed and proteins that interact with the TSWV G_N protein identified. These results provide a basis for further studies of the interaction between F. occidentalis and TSWV.