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八种常用农药与蜡蚧轮枝菌JMC-01的相容性及对烟粉虱若虫的毒力测定
Compatibility of 8 common pesticides with Lecanicillium lecanii JMC-01 and the toxicity of different pesticide- L. lecanii combinations toxicity to Bemisia tabaci nymphs
谢 婷;景亮亮;张晓霞;张 英;马 跃;姜 灵;王新谱;贾彦霞
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DOI:10.7679/j.issn.2095-1353.2020.069
作者单位:宁夏大学农学院;宁夏大学农学院;宁夏大学农学院;宁夏大学农学院;宁夏大学农学院;宁夏大学农学院;宁夏大学农学院;宁夏大学农学院
中文关键词:农药;蜡蚧轮枝菌JMC-01;烟粉虱;相容性;共毒系数
英文关键词:pesticide; Lecanicillium lecanii JMC-01; Bemisia tabaci; compatibility; co-toxicity coefficient
中文摘要:
【目的】 为明确8种常用农药与蜡蚧轮枝菌的相容性及对烟粉虱Bemisia tabaci若虫的毒力。   【方法】 该试验采用涂板法和浸渍法分别测定了8种常用农药对蜡蚧轮枝菌菌丝生长、孢子萌发和产孢量以及对烟粉虱若虫的LC50和共毒系数。【结果】 结果表明,随着农药稀释倍数的增加,蜡蚧轮枝菌菌丝生长抑制率、孢子萌发抑制率和产孢抑制率依次降低。其中,生物源农药乙基多杀菌素、藜芦碱、印楝素与蜡蚧轮枝菌的相容性和协同增效作用最好。乙基多杀菌素、藜芦碱、印楝素在10倍稀释浓度下,孢子萌发抑制率分别为20.02%、16.41%、15.38%;产孢抑制率分别为17.77%、15.90%、14.96%;菌丝生长抑制率分别为9.96%、8.87%、9.74%。蜡蚧轮枝菌与印楝素、藜芦碱混合后协同增效作用最好,CTC最大值分别为315和302。【结论】 因此,应用蜡蚧轮枝菌防治烟粉虱,选择相容性好、共毒系数高的农药以低剂量与蜡蚧轮枝菌复配使用,可以大幅度减少农药的用量,对温室大棚烟粉虱的生物防治提供参考依据。
英文摘要:
[Objectives]  To determine the compatibility of eight common pesticides with Lecanicillium lecanii JMC-01, and the toxicity of combinations of each pesticide and L. lecanii JMC-01 to Bemisia tabaci nymphs. [Methods]  The effects of each of eight pesticides on the spore germination and sporulation of L. lecanii, and their LC50 and co-toxicity coefficients with respect to B. tabaci nymphs, were tested using the coating and dipping methods. [Results]  The growth inhibition rate, spore germination inhibition rate and sporulation inhibition rate of L. lecanii decreased with pesticide dilution factor. The bio-pesticide lspinetoram, veratrine and azadirachtin were the most compatibile and had the best synergistic effect with L. lecanii. The inhibition rates of spore germination for 10-fold dilutions of lspinetoram, veratrine and azadirachtin were 20.02%, 16.41%, and 15.38%, respectively, and sporulation inhibition rates were 17.77%, 15.90%, and 14.96%, respectively. Mycelial growth inhibition rates for the above pesticides were 9.96%, 8.87%, and 9.74%, respectively. The best synergistic control was achieved using a combination of azadirachtin and veratrine with L. lecanii. Maximum CTC was 315 and 302, respectively. [Conclusion]  Selecting pesticides that are compatibile with L. lecanii, and that have a high co-toxicity coefficient at low doses, can significantly reduce the amount of pesticide required to control B. tabaci in greenhouses.
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