【目的】 明确橘园常用药剂对巴氏新小绥螨Neoseiulus barkeri成螨的致死效应，弄清巴氏新小绥螨甲氰菊酯抗性品系对柑橘园常用药剂的交互抗性水平及生态适合度变化，为巴氏新小绥螨抗性品系的田间应用提供科学理论依据。【方法】 在对巴氏新小绥螨进行致死效应和交互抗性测定的基础上，运用生态学方法对其生物学特性进行评价。【结果】 不同药剂对巴氏新小绥螨成螨致死效应存在显著差异。高效氯氟氰菊酯和毒死蜱的致死率最高，校正死亡率分别为97.62%和92.57%；巴氏新小绥螨甲氰菊酯抗性品系螺螨酯、噻虫嗪、乙螨唑、毒死蜱和高效氯氟氰菊酯均存在显著交互抗性，其抗性倍数分别为7.56、10.32、11.45、19.10和45.89倍。生物学特性研究结果表明，与敏感品系相比，甲氰菊酯抗性的获得使其发育历期显著延长，但对捕食量和孵化率影响不显著。哒螨灵、丁氟螨酯和高效氯氟氰菊酯对巴氏新小绥螨抗性品系与敏感品系卵的孵化率具有显著影响，其他常用药剂对巴氏新小绥螨抗性品系与敏感品系卵的孵化率不存在显著影响。【结论】 甲氰菊酯抗性获得使巴氏新小绥螨对柑橘园常用药剂表现不同水平的交互抗性；甲氰菊酯抗性获得对巴氏新小绥螨生长、繁殖及捕食均无显著影响，可在田间推广应用。

[Objectives]  To measure the cross-resistance of a fenpropathrin-resistant strain of the mite Neoseiulus barkeri to commonly used pesticides in citrus orchards and thereby provide guidance for the use of such strains in biological pest control. [Methods]  The lethality and cross-resistance of a fenpropathrin-resistant strain of N. barkeri to commonly used pesticides in orange orchards were measured and the biological characteristics of this evaluated. [Results]  There were significant differences in the lethality of different insecticides to adult N. barkeri. High-efficiency cyfluthrin and chlorpyrifos were the most lethal with corrected mortality rates of 97.62% and 92.57%, respectively. The fenpropathrin-resistant strain of N. barkeri had significant cross-resistance to spirodiclofen, thiamethoxam, ethimazole, chlorpyrifos and high-efficiency cyfluthrin, being 7.56, 10.32, 11.45, 19.10 and 45.89 times, respectively, more resistant to these pesticides than a susceptible strain. The developmental period of the resistant strain was significantly longer than that of the sensitive strain, but there were no significant differences in the prey capture and hatching rates of these strains. Pyridaben, bufomite and fenpropathrin had significant effects on the hatching rate of both resistant and sensitive strains of N. barkeri, whereas most of other common pesticides had no effect on hatch rate. [Conclusion]  Fenpropathrin-resistance was associated with different levels of cross-resistance to common pesticides in orange orchards but had no significant effect on the growth, reproduction and prey capture rate of N. barkeri, which could be more widely used as a biological control.