【目的】 明确棉蚜Aphis gossypii Glover体内3个高表达谷胱甘肽-S-转移酶（GST）基因不同发育阶段的表达谱，并比较它们在不同寄主专化型棉蚜中的表达差异。【方法】 从棉蚜的伏蚜和苗蚜转录组数据库中挑选GSTs并克隆得到全长cDNA序列，使用qRT-PCR分析这些基因在不同寄主专化型棉蚜不同龄期中的相对表达量。【结果】 克隆到3个高表达的GSTs基因，分别命名为AgoGST-s1（GenBank登录号：MN688789）、AgoGST-d1（GenBank登录号：MN688790）、AgoGST-d2（GenBank登录号：MN688791）。这3个基因在不同龄期均表达，不同发育阶段的表达谱基本一致，表达量呈现从低到高的变化趋势。AgoGST-s1基因在各个发育阶段相对表达量的变化幅度较小，而AgoGST-d1、AgoGST-d2的变化幅度较大。对比两种寄主专化型棉蚜相同龄期时的表达情况发现，黄瓜型棉蚜中GSTs基因的表达量在大部分龄期都高于棉花型棉蚜。【结论】 AgoGST-s1、AgoGST-d1、AgoGST-d2基因表达量伴随棉蚜生长发育变化趋势明显，在不同寄主专化型棉蚜体内的表达量存在差异，可能与棉蚜对寄主植物的适应性有关。

[Objectives]  To determine the profiles of three high expression glutathione-S-transferase (GST) genes in various developmental stages of Aphis. gossypii Glover, and compare the expression of these genes in different host-races of this pest. [Methods]  Full-length cDNA sequences of GSTs genes were cloned based on transcriptome data of A. gossypii specimens collected from seedling and mid-summer growth stages of cotton. The relative expression levels in different developmental stages (nymph and adult) and host-races (cucumber and cotton host-races) were investigated with real-time quantitative PCR. [Results]  Three high expression GSTs were identified and named AgoGST-s1 (GenBank accession no.: MN688789), AgoGST-d1 (GenBank accession no.: MN688790) and AgoGST-d2 (GenBank accession no.: MN688791). These 3 genes were expressed in all development stages and their temporal expression profiles all tended to increase over time. The relative expression of AgoGST-s1 gene was less in all developmental stages, whereas that of AgoGST-d1 and AgoGST-d2 was more variable. Expression of these 3 GSTs in the cucumber host-race was higher than in the cotton host-race for most age-classes. [Conclusion]  Expression of the AgoGST-s1, AgoGST-d1 and AgoGST-d2 genes is significantly associated with the developmental stage of A. gossypii. The expression patterns of these 3 GST genes in two host-races differed, which may reflect adaptation to different host plants.