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致死中浓度溴氰菊酯对茄无网蚜体内能源物质的影响
Effect of a lethal concentration of deltamethrin on the growth and nutrient content of Acyrthosiphon solani nymphs
高云雷;韩岚岚;赵奎军;张雯林;肖建飞;陈 娟;高丽瞳;邓士群
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DOI:10.7679/j.issn.2095-1353.2020.088
作者单位:东北农业大学农学院;东北农业大学农学院;东北农业大学农学院;东北农业大学农学院;东北农业大学农学院;东北农业大学农学院;东北农业大学农学院;东北农业大学农学院
中文关键词:茄无网蚜;致死中浓度;溴氰菊酯;生长历期;能源物质
英文关键词:Acyrthosiphon solani; lethal concentration; deltamethrin; instar sduration; energy substances
中文摘要:
【目的】 通过LC50溴氰菊酯胁迫1龄茄无网蚜Acyrthosiphon solani测定对其生长发育及能源物质的影响,为研究茄无网蚜抗药性产生提供理论基础。【方法】 通过浸渍法用LC50溴氰菊酯处理1龄茄无网蚜,间隔12 h观测药剂胁迫后试虫的生长历期,分别用蒽酮法、考马斯亮蓝G-250法,香草醛试剂测定各虫龄试虫蛋白、糖类和总脂质含量并进行数据分析。【结果】 药剂胁迫下显著延长了1龄(1.44倍)、2龄(1.26倍)的发育历期(P < 0.05);蛋白质比重在2龄(0.75倍)、3龄(0.69倍)、成蚜(1.17倍)有显著差异(P < 0.05),其他虫龄有差异但不显著;可溶性糖比重在2龄(1.39倍)差异显著(P < 0.05);糖原比重在1龄(3.81倍)、2龄(3.82倍)差异显著;总脂质在1-4龄均有显著差异(P < 0.05),分别是对照组的2.04、1.34、1.25、1.30倍。【结论】 药剂胁迫对茄无网蚜的生长发育及各虫龄能源物质比重均有不同程度的影响。
英文摘要:
[Objectives]  To investigate the effect of deltamethrin on the growth, development and energy storage of 1st instar nymphs of Acyrthosiphon solani Kaltenbach and thereby provide a theoretical basis for research pesticide resistance mechanisms in this pest. [Methods]  First instar nymphs of A. solani were treated with an LC50 dose of deltamethrin via the impregnation method after which the growth of successive instars was monitored every 12 h. In addition, the protein, carbohydrate and total lipid of each nymph was measured by staining these with anthrone, coomassie brilliant blue G-250 and vanillin, respectively. [Results]  We found that deltamethrin significantly extended the growth period of 1st and 2nd instar nymphs by 1.44 times and 1.26 times, respectively (P < 0.05). There was also a significant difference in the specific gravity of protein between treatment and control groups in 2nd (0.74 times), 3nd (0.69 times) and 5th instar (1.17 times) nymphs. The protein content of other treated instars also differed from their respective control group, but not significantly. There was also a significant difference in the specific gravity of soluble carbohydrate between 2nd instar treatment and control groups  (1.39 times). Glycogen differed significantly between treatment and control groups in the 1st (3.81 times) and 2nd instars  (3.82 times). Finally, total lipid content significantly differed between the treatment and control groups of all four instars; treatment groups having 2.04, 1.34, 1.25 and 1.30 times the total lipid content of their respective control groups. [Conclusion]  An LC50 dose of deltamethrin effects both the growth and nutrient content of all instars of A. solani.
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