【目的】 磷酸甘油酸变位酶（PGAM）是糖酵解和葡萄糖异生途径中一种发挥重要作用的蛋白酶，本研究拟明确amPGAM2基因的序列特征及表达模式。【方法】 以意大利蜜蜂Apis mellifera为研究对象，克隆了amPGAM2基因的cDNA序列，分析了其序列特征及其在工蜂、雄蜂、蜂王的不同发育时期的表达模式。【结果】 序列特征分析表明，克隆所得序列全长976 bp，包含1个完整的开放阅读框，共编码254个氨基酸。该基因核苷酸序列与中华蜜蜂Apis cerana（98.4%）高度相似，并存在15个潜在抗原表位、9个磷酸化位点和5个组氨酸磷酸酶域活性部位，属于组氨酸磷酸酶超家族，是一种二磷酸甘油酸依赖性的可溶中性稳定蛋白。荧光定量PCR结果表明，amPGAM2基因在不同品级、不同发育时期的表达差异显著（P<0.05）。工蜂卵期3日龄、幼虫期5日龄表达最高，雄蜂成虫期表达最高，蜂王幼虫期4日龄表达最高，且工蜂、雄蜂及蜂王由卵孵化成幼虫阶段和由红眼蛹羽化至成虫阶段，其表达都呈上升趋势。【结论】 本研究结果推测amPGAM2基因在卵的孵化及精卵发生过程中具有重要作用，为进一步认识意大利蜜蜂生殖发育过程中的能量代谢提供理论基础。

[Objectives]  Phosphoglycerate mutase (PGAM) plays an important role in the glycolysis and gluconeogenesis of protease. This study intends to clarify the sequence characteristics and expression patterns of the amPGAM2 gene. [Methods]  The cDNA sequence of the Apis mellifera amPGAM2 gene was cloned, and its sequence characteristics and expression in different castes and developmental stages; workers, drones and queens, analyzed. [Results]  Sequence analysis indicates that the full length of the cloned sequence is 976 bp, and contains a complete open reading frame encoding 254 amino acids. The nucleotide sequence of the gene is highly homologous to that of Apis cerana (98.4%). The gene has 15 potential antigen epitopes, 9 phosphorylation sites and 5 histidine phosphatase domain active sites and encodes a glycerol diphosphate-dependent soluble neutral stable protein that belongs to the histidine phosphatase superfamily. The results of RT-qPCR indicate that expression of amPGAM2 is significantly different in different castes and developmental stages. Expression in workers was highest at 3 days of age in the egg stage and at 5 days in the larval stage. The highest expression in drones and queesn was in the adult stage and in 4 day-old larvae, respectively. Expression in workers, drones and queens increased from hatching to the larval stage, and from the red-eye pupal eclosion to the adult stage. [Conclusion]  The results suggest that the amPGAM2 gene plays an important role in hatching and spermatogenesis. This paper provides a theoretical basis for improving understanding of the energy metabolism during reproductive development in Apis mellifera.