草地贪夜蛾CYP4G15和CYP4L4的时空表达及其对高低温胁迫的响应
Spatiotemporal expression of CYP4G15 and CYP4L4 and their response to high and low temperature stress in Spodoptera frugiperda
商 暾 杨茂发 于晓飞 陈亦钗 田太安
点击：718次 下载：46次
DOI：10.7679/j.issn.2095-1353.2021.067
作者单位：贵州大学昆虫研究所，贵州山地农业病虫害重点实验室，贵阳 550025；贵州大学烟草学院，贵阳 550025
中文关键词：草地贪夜蛾；温度胁迫；CYP4G15；CYP4L4；差异表达
英文关键词：Spodoptera frugiperda; temperature stress; CYP4G15; CYP4L4; differential expression
中文摘要：
【目的】 为明确草地贪夜蛾Spodoptera frugiperda在高低温胁迫条件下CYP4G15和CYP4L4是否参与调控。【方法】 本研究采用RT-PCR方法检测CYP4G15和CYP4L4在草地贪夜蛾各发育阶段和不同组织以及在36 ℃高温和4 ℃低温条件下的表达情况。【结果】 CYP4G15和CYP4L4基因均在草地贪夜蛾低龄时表达量最高；CYP4G15在表皮组织中表达量最高；CYP4L4在前肠组织中表达量最高；在高温和低温胁迫条件下，两个基因均存在差异表达。【结论】 CYP4G15和CYP4L4可能参与草地贪夜蛾体内多种内源物质合成以及受高温、低温胁迫的代谢过程。
英文摘要：
[Objectives] To explore whether CYP4G15 and CYP4L4 are involved in the
response to high and low temperature stress in Spodoptera frugiperda. [Methods] The expression of CYP4G15 and CYP4L4 were detected by RT-PCR at 36 ℃ and 4 ℃ in different developmental
stages and tissues. [Results]
Expression of both genes was highest in young larvae. Expression of CYP4G15 was highest in the epidermis, whereas that of CYP4L4 was highest in the
foregut. The two genes were differentially expressed at high and low
temperatures. [Conclusion] CYP4G15 and CYP4L4 may be involved in the synthesis of various endogenous substances
in S. frugiperda, as well as metabolic processes under high and low
temperature stress.