六株冬虫夏草相关菌对蝙蝠蛾幼虫侵染特性的研究
Relative infectiveness of six Cordyceps sinensis-related fungal strains to Hepialus armoricanus larvae
谢 放 陈照禾 朱玉兰 苏强军 陈 晨 张 育
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DOI：10.7679/j.issn.2095-1353.2021.131
作者单位：兰州交通大学生物与制药工程学院，兰州 730070；兰州交通大学生物与制药工程学院，兰州 730070；兰州交通大学生物与制药工程学院，兰州 730070；兰州交通大学生物与制药工程学院，兰州 730070；兰州交通大学生物与制药工程学院，兰州 730070；兰州交通大学生物与制药工程学院，兰州 730070
中文关键词：冬虫夏草相关菌；蝙蝠蛾幼虫；冬虫夏草菌；侵染
英文关键词：Cordyceps sinensis-related fungus; Hepialus armoricanus larvae; Ophiocordyceps sinensis; infection
中文摘要：【目的】 本文将冬虫夏草相关菌定义为分离自野生冬虫夏草Cordyceps sinensis，可以反接侵染蝙蝠蛾Hepialus armoricanus幼虫致死及与冬虫夏草菌Ophicordyceps
sinensis对峙有相互影响的菌株。本文旨在对分离自冬虫夏草不同部位的6株相关菌单独或与冬虫夏草菌协同侵染蝙蝠蛾幼虫的效应进行研究。【方法】 对6株相关菌进行生物学特征描述，并通过ITS序列比对进行种属鉴定；对6株相关菌做了与冬虫夏草菌的对峙实验及单独侵染蝙蝠蛾幼虫的实验；对其中的2株菌做了与冬虫夏草菌复合侵染蝙蝠蛾幼虫实验。【结果】 证明了6株相关菌能够影响冬虫夏草菌的生长，其中菌株2F-2、FD、TB-1和TB-4对冬虫夏草菌抑制率接近100%，菌株3-5-2对冬虫夏草菌抑制率为4.32%；菌株MR-1对冬虫夏草菌抑制率为77.27%；6株相关菌均有侵染蝙蝠蛾幼虫的能力：菌株3-5-2对蝙蝠蛾幼虫侵染率为50.0%，菌株2F-2对蝙蝠蛾幼虫侵染率为85.0%，菌株MR-1对蝙蝠蛾幼虫侵染率为73.3%，菌株FD对蝙蝠蛾幼虫侵染率为70.0%，菌株TB-1对蝙蝠蛾幼虫侵染率为64.0%，菌株TB-4为65.0%；相对于单独侵染，复合侵染致死率有所下降。【结论】 供试的6株相关菌均有侵染力并与冬虫夏草菌相互影响。
英文摘要：
[Objectives] To study the relative infectiousness of six fungal strains isolated from
different parts of Cordyceps sinensis to Hepialus armoricanus larvae. C. sinensis-related fungi are defined as
fungi isolated from wild C. sinensis that can infect and kill H. armoricanus larvae and interact with O. sinensis. [Methods] Biological characteristics of 6 strains of
related fungi were described and their ITS sequences were aligned to identify
the species. The infectiveness of the six strains of related fungi to H. armoricanus larvae were tested
separately. Two related fungi were tested in a joint infection experiment with O. sinensis. [Results] The 6 strains of related fungi all affected the growth of O. sinensis: The inhibition rate of the
2F-2, FD, TB-1 and TB-4 strains to O.
sinensis was close to 100%, that of the 3-5-2 strain was 4.32% and that of
the MR-1 strain was 77.27%. All six strains had the ability to infect H. armoricanus larvae. The infection rate of the 3-5-2 strain was 50.0%, that
of the 2F-2 strain was 85.0% and that of the MR-1 strain was 73.3%. The
infection rate of FD strain was
70.0%; the infection rate of the TB-1 strain was 64.0% and the infection rate
of the TB-4 strain was 65.0%. The mortality rate of
joint infections was lower than that of single-strain infections. [Conclusion] The 6 strains of related fungi were all infectious to H. armoricanus larvae and can interact with O.
sinensis.