米蛾嗅觉基因的转录组分析
Analysis of the olfactory genes of Corcyra cephalonica (Lepidoptera: Pyralidae)
李鹏燕 张秋婷 黄铮昱 黄 华 陈伟平 廖永林
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DOI：10.7679/j.issn.2095-1353.2021.135
作者单位：广东省农业科学院植物保护研究所，广东省植物保护新技术重点实验室，广州 510640； 中南林业科技大学食品科学与工程学院，稻谷及副产物深加工国家工程实验室，长沙 410004
中文关键词：米蛾；转录组测序；嗅觉基因；代谢通路；功能注释
英文关键词：Corcyra cephalonica; transcriptome sequencing; olfactory genes; function annotation
中文摘要：
【目的】 建立米蛾Corcyra cephalonica成虫转录组数据库，获得米蛾的嗅觉基因信息。【方法】 采用高通量测序平台（Illumina Hiseq）对米蛾进行转录组测序，用Trinity软件对原始数据进行组装分析，在7个数据库中用Blast软件对unigene进行注释，用PCR方法检测米蛾信息素结合蛋白基因（Pheromone binding protein，PBPs）和普通气味结合蛋白基因（General odorant binding
protein，GOBPs）在成虫不同组织中的表达情况。【结果】 最终获得58 534条unigene，其中25 879条得到注释；N50为2 212 bp，平均长度1 251 bp；与脐橙螟Amyelois transitella相似度最高（28.2%）；发掘了20个气味结合蛋白（Odorant binding proteins,
OBPs），18个化学感受蛋白（Chemosensory proteins, CSPs），2个GOBPs和3个PBPs，均已上传NCBI。组织表达分析发现PBPs和GOBPs基因都在米蛾成虫触角上大量表达，在头、胸、腹、足、翅中少量表达，PBP3在成虫胸部组织中无表达，GOBP2仅在成虫触角上大量表达，在其他组织中无表达。【结论】 研究结果丰富和完善了米蛾的嗅觉基因信息，为米蛾的嗅觉识别机制研究和诱剂研发奠定了分子基础。
英文摘要：
[Objectives] To obtain olfactory
gene information and establish a transcriptome database for the adult rice
moth, Corcyra cephalonica. [Methods] The C.
cephalonica transcriptome was sequenced on the Illumina HiSeq
platform, the original data were assembled and analyzed using the Trinity
software package and Blast was used to annotate unigenes in 7 databases. The
expression of pheromone binding proteins (PBPs) and general odorant binding
proteins (GOBPs) in different adult moth tissues was then detected using PCR. [Results] A total of 58 534 unigenes
were obtained, 25 879 of which were annotated with an N50 value of 2 212 bp and
mean length of 1 251 bp. C.
cephalonica unigenes had highest similarity (28.2%) to those of Amyelois transitella; 20 odorant binding
proteins (OBPs) genes, 18 chemosensory proteins (CSPs) genes, 2 general odorant
binding protein (GOBPs) genes and 3 pheromone binding proteins
(PBPs) genes, were identified and uploaded to NCBI. PBPs and GOBPs were highly
expressed in the antennae of adult moths but expression was lower in the head,
thorax, abdomen, leg and wing. PBP3 was not expressed in the thorax, and GOBP2
was only highly expressed in the antennae and not in other tissues. [Conclusion] The results significantly increase the
available information on the olfactory genes of C. cephalonica, and provide a molecular foundation for the
study of olfactory recognition mechanisms and the development of attractants for
this pest.