小线角木蠹蛾幼虫适应不同寄主的 转录组学分析
Transcriptomics analysis of Streltzoviella insularis staudinger larvae (Lepidoptera, Cossidae) adapting to different hosts
王秀吉 冯宇倩 续亚贝 宗世祥
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DOI:10.7679/j.issn.2095-1353.2022.038
作者单位:林业有害生物防治北京市重点实验室,北京 100083;中国林业科学研究院林业新技术研究所, 森林病原整合生物学研究室,北京 100091
中文关键词:小线角木蠹蛾;寄主适应性;转录组;酶
英文关键词:Streltzoviella insularis; host adaptation; transcriptome; enzymes
中文摘要:
【目的】 通过比较小线角木蠹蛾Streltzoviella insularis Staudinger幼虫取食不同寄主后的肠道转录组,旨在挖掘幼虫对寄主适应的相关基因,为探究小线角木蠹蛾幼虫对寄主的适应机制提供分子生物学数据。【方法】 采用Illumina二代测序技术,测定小线角木蠹蛾幼虫取食3种不同寄主树种(洋白蜡、银杏和国槐)15 d后的肠道转录组,使用BLAST软件对unigene序列进行GO和KEGG富集,通过比较分析筛选出幼虫对寄主适应的相关基因。【结果】 取食国槐与取食洋白蜡的小线角木蠹蛾幼虫的差异表达基因数量有916个,取食银杏与取食洋白蜡的小线角木蠹蛾幼虫的差异表达基因数量为1 163个,取食银杏与国槐的小线角木蠹蛾幼虫的差异表达基因数量为1 621个;其中,上调表达的差异基因与解毒代谢、水解酶和转运蛋白等密切相关;取食不同寄主的幼虫肠道差异基因中,解毒酶(17个)和消化酶(20个)的差异基因数量都明显多于保护酶(5个)。本研究筛选出2个解毒酶基因、3个消化酶基因和1个保护酶基因,可作为小线角木蠹蛾幼虫对寄主适应机制的重点研究对象。【结论】 本研究初步揭示了小线角木蠹蛾幼虫响应不同寄主的差异表达基因,为进一步探究小线角木蠹蛾对寄主的适应机制提供了基础依据。
英文摘要:
[Objectives] By comparing the gut transcriptome of Streltzoviella insularis Staudinger larvae feeding on
different hosts, the aim is to explore the genes related to the host adaptation
of S. insularis larvae. It will
provide molecular biological data for exploring the adaptation mechanism of S. insularis larvae to the host. [Methods] The Illumina second-generation sequencing
technology was used to determine the transcriptome of the S. insularis gut, after the larvae were fed on three different
hosts (Fraxinus pennsylvanica, Ginkgo biloba, Sophora japonica) 15 days. BLAST software were performed GO and
KEGG enrichment on unigene sequences. The
genes related to host adaptation of larvae were screened by comparative
analysis. [Results] There were 916 differentially expressed
genes between the larvae feeding on S.
japonica and the larvae feeding on F.
pennsylvanica, 1 163 differentially expressed genes between the larvae
feeding on G. biloba and the larvae
feeding on F. pennsylvanica, and 1
621 differentially expressed genes between the larvae feeding on G. biloba and the larvae feeding on S. japonica. The up-regulated
differential genes were closely related to detoxification metabolism, hydrolase
and transporter proteins. The number of differentially expressed genes
annotated to detoxification enzymes (17 genes) and digestive enzymes (20 genes)
was significantly higher than those to protective enzymes (5 genes) in larvae
fed on different hosts. Two detoxification enzymes genes, three digestive
enzyme genes and a protective enzyme gene were screened out as the key genes
for the study of S. insularis larval
adaptation to hosts. [Conclusion] This study initially revealed the
differentially expressed genes of S.
insularis larvae in response to different hosts. It will provide a basis
for further exploration of the adaptation mechanism of S. insularis larvae to hosts.