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取食蚜害后的棉花对棉蚜营养代谢的影响
Effects of aphid damage to cotton plants on the nutritional metabolism of Aphis gossypii
王小丽;吴 娜;张玉栋;韩 睿;张全成;王俊刚
点击:454次 下载:14次
DOI:10.7679/j.issn.2095-1353.2022.089
作者单位:石河子大学农学院;新疆绿洲农业病虫害治理与植保资源利用重点实验室
中文关键词:棉长管蚜;棉蚜;营养代谢;交互取食
英文关键词:Acyrthosiphon gossypii; Aphis gossypii; nutrition metabolism; interactive feeding
中文摘要:
【目的】 为明确棉蚜Aphis gossypii Glover取食受蚜虫为害的棉花后,其体内营养代谢的变化。【方法】 采用生化分析法,研究取食棉长管蚜Acyrthosiphon gossypii Mordviiko持续为害以及棉长管蚜和棉蚜交互为害的棉花对棉蚜体内淀粉酶、海藻糖酶、蔗糖酶、脂肪酶和胃蛋白酶活性的影响。【结果】 与取食健康棉花相比,取食经棉长管蚜为害72 h的棉花后,棉蚜体内的淀粉酶、海藻糖酶和胃蛋白酶活性分别降低13.0%、13.7%和2.9%,蔗糖酶和脂肪酶活性分别上升13.6%、11.1%。与取食棉长管蚜为害72 h的棉花相比,取食经棉长管蚜-棉蚜-棉长管蚜交互为害72 h的棉花,棉蚜体内淀粉酶、海藻糖酶、胃蛋白酶、蔗糖酶和脂肪酶活性分别下降10.0%、3.5%、0.5%、3.5%和6.5%。棉花在相同的蚜虫为害时间以及为害次序下,对后期取食的棉蚜消化酶活性的影响也各不相同。与经棉长管蚜、棉蚜和棉长管蚜分别交互为害72、24和24 h处理的棉花相比,取食经棉长管蚜、棉蚜和棉长管蚜分别交互为害24、72和24 h处理的棉花棉蚜体内淀粉酶活性下降23.5%、海藻糖酶活性下降7.3%,蔗糖酶和脂肪酶活性分别上升14.4%和8.0%。而与经棉长管蚜、棉蚜和棉长管蚜分别交互为害24、24和72 h处理的棉花相比,取食经棉长管蚜、棉蚜和棉长管蚜分别交互为害24、72和24 h处理的棉花棉蚜体内胃蛋白酶活性上升43.1%。【结论】 棉长管蚜为害棉花可导致后期取食的棉蚜体内淀粉酶、海藻糖酶以及胃蛋白酶活性受到抑制,而棉蚜则会通过蔗糖酶和脂肪酶活性的升高来弥补因淀粉酶、海藻糖酶及胃蛋白酶活性受到抑制而导致的营养物质吸收不足。相同的虫害处理时间以及处理次序下,由于棉蚜的加入使得虫害棉花对后取食的棉蚜体内消化酶活性的影响更加显著。
英文摘要:
[Objectives]  To clarify changes in the nutritional metabolism of Aphis gossypii after feeding on cotton plants stressed by aphid damage. [Methods]  Biochemical methods was used to study the effects of continuous damage by Acyrthosiphon gossypii, and the interaction between Acyrthosiphon gossypii and the Aphis gossypii, on the amylase, trehalase, invertase, lipase and pepsin levels of Aphis gossypii that subsequently fed on the same plants. [Results]  The amylase, trehalase and pepsin activity of Aphis gossypii that had fed on cotton damaged by Acyrthosiphon gossypii for 72 h decreased by 13.0%, 13.7% and 2.9%, respectively, and their sucrase and lipase activity increased by 13.6% and 11.1%, respectively, compared to the activity of these enzyme in aphids that had fed on healthy cotton. Amylase, trehalase, pepsin, sucrase and lipase activity decreased by 10.0%, 3.5%, 0.5%, 3.5% and 6.5%, respectively, when Aphis gossypii had fed on cotton that had been first been fed on by Acyrthosiphon gossypii, then by Aphis gossypii, then again by Acyrthosiphon gossypii, for 72 h. The effects of the same duration of aphid damage, and the same sequential order of aphid species, on the digestive enzyme activity of Aphis gossypii differed when these fed at a later stage. The amylase and trehalase activity of aphids that fed on plants treated with Acy24A72Acy24 was 23.5% and 7.3% lower, respectively, than those that had fed on cotton plants treated with Acy72A24Acy24, whereas invertase and lipase activity increased by 14.4% and 8%, respectively. However, the pepsin activity of Aphis gossypii fed on cotton plants treated with Acy24A72Acy24 was 43.1% higher than that of those that had fed on plants treated with Acy24A24Acy72. [Conclusion]  Amylase, trehalase and pepsin activity in Aphis gossypii were inhibited by the previous feeding activity of Acyrthosiphon gossypii on cotton plants, and sucrase and lipase activity increased to compensate for the reduced absorption of nutrients caused by the inhibition of amylase, trehalase and pepsin. The same treatment time and order of species had a more significant effect on the digestive enzyme activity of Aphis gossypii.
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