水稻害虫代谢组学研究概况与展望
Current progress and prospects for future research on the rice pest metabolomes
崔艺琳；俞晓平；李丹婷
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DOI：10.7679/j.issn.2095-1353.2022.098
作者单位：中国计量大学生命科学学院，浙江省生物计量及检验检疫技术重点实验室，杭州 310018；
中文关键词：水稻害虫；代谢组学；研究方法；多组学联合分析；展望
英文关键词：rice pests; metabolomics; research methods; multi-omics combined analysis; prospect
中文摘要：
水稻作为主要的经济作物之一，在种植过程中不断受到多种水稻害虫的侵害，极大地影响了人类农业生产和粮食安全。代谢组学分析不但可以反映细胞或生物体内的生理生化变化，而且可以分析生物体受到不同因素影响时的代谢变化规律。本文简要综述了代谢组学在水稻害虫研究中的进展，介绍了代谢组学的主要研究方法，即核磁共振技术和质谱色谱联用技术，总结了代谢组学在水稻害虫研究中的应用，包括昆虫抗药性研究、昆虫-水稻相互作用研究及昆虫-共生菌相互作用研究等，同时分析了目前代谢组学分析在准确度、灵敏度及分辨率等各方面存在的不足，以及代谢组学等多组学联合分析技术在昆虫研究领域的应用前景，为寻找新型杀虫靶标、开发新型生物农药和转基因抗性水稻等奠定基础，同时也为水稻害虫的绿色防控技术提供新的突破点。
英文摘要：Rice crops are constantly damaged by a variety
of pests during cultivation, which greatly affects human food security.
Metabonomic data can not only reflect internal physiological and biochemical
changes in cells or organisms, but also indicate how these are affected by
different factors. This paper reviews progress in research on the metabolomes
of rice pests, introduces the main methods used in metabolomics, i.e., nuclear magnetic resonance technology and mass spectrometry
chromatography coupling technology, and summarizes the application of metabolomics to rice pest research, including that on
pesticide resistance, insect-rice interactions and insect-symbiotic bacteria
interactions. The shortcomings of metabolomics in terms of accuracy, sensitivity and resolution are also discussed, as
well as prospects for using metabolomics and other combined multi-omics
techniques to find novel insecticidal targets, develop new
biological pesticides and transgenic resistant rice plants, and other
environmentally-friendly methods for the prevention and control of rice pests.