稻纵卷叶螟ABCG3转运蛋白的分子特性与基因表达谱分析
Molecular characteristics and gene expression of the Cnaphalocrocis medinalis (Guenée) ABCG3 transporter protein
吕 柯；何晓婵；徐红星；吕仲贤；杨亚军
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DOI：10.7679/j.issn.2095-1353.2022.101
作者单位：农产品质量安全危害因子与风险防控国家重点实验室，农业农村部植保生物技术重点实验室，浙江省农业科学院植物保护 与微生物研究所，杭州 310021；南京农业大学植物保护学院，南京 210095；金华市农业科学研究院，金华 321000
中文关键词：稻纵卷叶螟；ABC转运蛋白；蛋白结构；表达分析
英文关键词：Cnaphalocrocis medinalis (Guenée); ABCG transporter; protein structure; expression analysis
中文摘要：
【目的】 三磷酸腺苷结合盒转运子（ATP-binding cassette transporters，ABC转运蛋白）是广泛存在于各生物体内的一类跨膜蛋白，参与生物体内营养物质及外源毒物的转运。明确稻纵卷叶螟Cnaphalocrocis medinalis ABCG3的分子特性与表达谱特征，是开展该基因功能研究的基础。【方法】 利用生物信息学方法分析稻纵卷叶螟ABCG3转运蛋白的分子特性，利用定量PCR技术分析了其时空表达谱。【结果】 稻纵卷叶螟ABCG3含有一个跨膜结构域（TMD）和核酸结合结构域（NBD），属于半转运蛋白，包含1 055个氨基酸，分子量和等电点分别为118.95 ku和8.78，表达位置位于内质网。稻纵卷叶螟ABCG3蛋白包含43.51%的α-螺旋、7.01%的β-转角、18.29%的片层和31.18%的无规则卷。通过建立稻纵卷叶螟ABCG3与其他5种鳞翅目昆虫的ABCG亚家族系统发育进化树，发现稻纵卷叶螟ABCG3与黑脉金斑蝶Danaus plexippu亲缘关系最为接近。稻纵卷叶螟ABCG3基因在各龄期中及在前肠、中肠、后肠、血淋巴和脂肪体中均有表达，其中在5龄幼虫中的表达量高于其他龄期，血淋巴和脂肪体中的表达量较高。【结论】 这对于阐明稻纵卷叶螟ABC转运蛋白家族的结构特征和表达模式具有重要意义，为今后稻纵卷叶螟ABC转运蛋白的功能研究奠定基础。
英文摘要：
[Objectives] To improve understanding of the
characteristics and expression profile of the Cnaphalocrocis medinalis ABCG3 protein, one of
the ATP-binding cassette transporter (ABC transporter) transmembrane proteins
found in many organisms that is involved in the transport of nutrients and
exogenous toxins. [Methods] We used bioinformatics to
investigate the molecular properties of the C. medinalis ABCG3
transporter protein, and RT-qPCR to determine its expression patterns in
different larval instars and tissues. [Results] C. medinalis ABCG3 is a
half-transporter protein with 1 055 amino acids, a molecular weight of 118.95
ku and an isoelectric point of 8.78. It has a transmembrane domain (TMD) and a nucleic
acid binding domain (NBD). The protein is predicted to be located in the
endoplasmic reticulum, and has a 43.51% α-helix, a 7.01% β-angle, a 18.29%
lamellae and a 31.18% irregular roll. A phylogenetic tree of C. medinalis ABCG3 and that of five other lepidopteran species suggests that C. medinalis ABCG3 is most closely related to that of the monarch butterfly (Danaus
plexippus). The ABCG3 gene was found to be expressed in all larval
instars and tissues that were investigated. Expression was higher in fifth
instar larvae than in other instars, and higher in the hemolymph and fat body than
in other tissues. [Conclusion] These
findings clarify the structural properties and expression patterns of the ABC
transporter family in C. medinalis, and thereby provide a platform for further
investigation of the function of ABC transporters in this species.