褐飞虱CYP6ER1的时空表达及其对呋虫胺的响应
Spatiotemporal expression of CYP6ER1 and response to dinotefuran stress in Nilaparvata lugens
肖汉祥；李燕芳；陈珊媛；戴阳朔；袁龙宇；张振飞
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DOI：10.7679/j.issn.2095-1353.2022.107
作者单位：广东省农业科学院植物保护研究所，广州 510640；广东省植物保护新技术重点实验室，广州 510640
中文关键词：褐飞虱；抗药性；CYP6ER1；基因表达
英文关键词：Nilaparvata lugens; pesticide resistance; CYP6ER1; differential expression
中文摘要：
【目的】 揭示褐飞虱Nilaparvata lugens对呋虫胺抗药性形成的分子机制。【方法】 以呋虫胺不同抗性的褐飞虱种群为材料，采用qRT-PCR 方法检测CYP6ER1在褐飞虱各发育阶段和不同组织以及在呋虫胺半致死浓度处理后的P450酶的活性和CYP6ER1基因的表达情况。采用生物测定的方式检测了广东省不同地理种群褐飞虱对呋虫胺的抗药性和CYP6ER1基因在不同地理种群中的表达量。【结果】 CYP6ER1基因在抗性褐飞虱种群中表达量显著高于敏感种群，在成虫期3-9 d时和中肠组织中也有很高的表达量；与对照组相比，褐飞虱在呋虫胺半致死浓度处理后，CYP6ER1基因表达量和P450酶的活性均存在差异；而且广东地区褐飞虱不同地理种群CYP6ER1基因表达量和抗性水平有关。【结论】 CYP6ER1可能参与褐飞虱对呋虫胺的解毒代谢过程，影响褐飞虱对呋虫胺的抗药性。
英文摘要：
[Objectives] To investigate the molecular
mechanism responsible for the resistance of Nilaparvata
lugens to dinotefuran. [Methods] The expression of the CYP6ER1 gene and the activity of P450 enzymes were measured and
compared in brown planthopper (BPH) populations with different levels of
resistance to dinotefuran. The expression of the CYP6ER1 gene in different developmental stages and tissues and the
activity of P450 enzymes, were measured after treatment with semi-lethal
concentrations of dinotefuran. Susceptibility to dinotefuran was tested using
the stem-dip method in a laboratory. [Results] The CYP6ER1 gene was significantly more highly expressed in resistant than in sensitive,
populations. It was also more highly expressed in 3 to 9 day-old adults than in
other age groups, and in midgut tissue than in other tissues. BPHs treated with
semi-lethal concentrations of dinotefuran had significantly different CYP6ER1 gene expression and P450 enzyme
activity to the control group. [Conclusion] There is a correlation between expression of
the CYP6ER1 gene and resistance in
different N. lugens populations. CYP6ER1 may therefore be involved in the
detoxification of dinotefuran in the brown planthopper.