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不同密度黑尾叶蝉取食不同时长后水稻生理生化指标的响应
Biochemical and physiological response of rice plants induced by the feeding of Nephotettix cincticeps
赵文华;杨光梅;刘雨芳
点击:431次 下载:28次
DOI:10.7679/j.issn.2095-1353.2022.110
作者单位:湖南科技大学生命科学与健康学院,湘潭 411201;湖南科技大学生命科学与健康学院,湘潭 411201
中文关键词:黑尾叶蝉;取食胁迫;叶绿素;总蛋白;防御蛋白酶
英文关键词:Nephotettix cincticeps; feeding stress; chlorophyll; total protein; defense protease
中文摘要:
【目的】 探索不同密度黑尾叶蝉Nephotettix cincticeps取食不同时长后水稻Oryza sativa L.重要生理生化指标的变化。【方法】 设置黑尾叶蝉密度为0(对照)、2、4和8头/株4个处理,分别取食6、24、48和96 h后,检测水稻叶绿素(Chlorophyll,Chl)与总蛋白(Total protein,TP)含量,比较不同取食时间后,水稻脂氧合酶(Lipoxygenase,LOX)、总超氧化物歧化酶(Total superoxide dismutase,T-SOD)、过氧化物酶(Peroxidase,POD)和多酚氧化酶(Polyphenol oxidase,PPO)活性。【结果】 不同密度的黑尾叶蝉取食不同时长的水稻后,均极显著地降低水稻叶片的叶绿素含量(P˂0.01);取食6 h后,水稻叶片的蛋白质含量升高,且随着取食时间的延长,蛋白质含量显著降低(P˂0.05)。不同密度的黑尾叶蝉取食后,总体趋势是显著降低水稻LOX活性,但密度为2头/株的黑尾叶蝉取食6 h后,引起水稻LOX活性升高。不同密度的黑尾叶蝉取食24 h,均引起水稻PPO活性显著升高(P˂0.05)。水稻T-SOD活性随着黑尾叶蝉取食胁迫时间呈现动态变化,且与密度有关。不同密度的黑尾叶蝉取食刺激,导致水稻POD活性升高,黑尾叶蝉密度越高,POD活性升高越快,且密度为2头/株和4头/株的黑尾叶蝉取食96 h后,水稻POD活性极显著升高(P˂0.01)。【结论】 不同密度的黑尾叶蝉取食不同时长,均引起水稻产生明显的生理生化响应,表现为水稻Chl含量及LOX、PPO、T-SOD和POD酶活性的明显变化。
英文摘要:
[Objectives]  To explore the physiological and biochemical responses of rice to the feeding activity of the green rice leafhopper (GRL), Nephotettix cincticeps (Uhler) (Hemiptera: Cicadellidae). [Methods]  Physiological and biochemical indices of rice plants were measured at three GRL densities (2, 4, and 8 GRLs per plant). Rice Chl and TP content, LOX, T-SOD, POD and PPO activities were measured after GRLs had fed on plants for 6, 24, 48 and 96 h. The control group was comprised of GRL-free plants. [Results]  Chl content of all three density treatments was significantly lower than that of the control group (P<0.01). TP content increased after 6 hours of feeding, then significantly decreased as the duration of feeding increased (P<0.05). LOX activity in the 2 GRLs per plant treatment group significantly increased after 6 hours of feeding (P<0.05), then significantly decreased as the duration of feeding increased. PPO activity significantly increased after 24 hours of feeding in all three density treatment groups (P<0.05). T-SOD activity changed dynamically with the duration of feeding and GRL density. POD activity increased earlier at higher GRL densities; POD activity of the 2 and 4 GRLs per plant treatment groups was significantly higher than that of the control after 96 hours (P<0.051). [Conclusion]  Significant physiological and biochemical responses, including changes in Chl content, and in LOX, PPO, T-SOD and POD activity, were induced in rice plants by the feeding activity of N. cincticeps. These responses varied with GRL density and the duration of feeding time.
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