【目的】 明确性别、日龄及温度等因素对美国白蛾Hyphantria cunea (Drury)飞行能力的影响，为预测种群扩散趋势以及监测预警提供科学依据。【方法】 利用昆虫飞行磨吊飞方法，测定了不同日龄、性别及温度对美国白蛾成虫飞行能力的影响。【结果】 美国白蛾雄虫的飞行能力显著高于雌虫。不同日龄美国白蛾飞行能力也存在显著差异。雄虫的飞行能力随日龄的增加呈下降趋势。1日龄雄虫最大累计飞行距离17.87 km，最大累计飞行时间17.38 h。雌虫飞行能力随着日龄的增加呈现先增强后减弱的趋势，其中，3日龄雌虫最大累计飞行距离8.21 km，最大累计飞行时间7.4 h。对平均累计飞行距离、平均累计飞行时间以及平均飞行速度进行双因素方差分析，未发现明显的性别日龄交互作用。处于低温（16 ℃）环境条件下，雄虫飞行速度明显减缓。【结论】 美国白蛾雄虫飞行能力高于雌虫，随着日龄的增加，雄虫飞行能力逐渐降低，雌虫呈现出先升高后降低的变化趋势。低温对美国白蛾雄虫飞行能力具有一定影响。

[Objectives]  To clarify the influence of sex, age (days) and temperature, on the flight ability of adult Hyphantria cunea, and there by provide information essential for monitoring, and predicting, the dispersal of this pest. [Methods]  The effect of gender, age and temperature on the flight ability of adult H. cunea was measured in an insect flight mill. [Results] Males were significantly stronger fliers than females with a maximum flight distance of 17.87 km and cumulative flight time of 17.38 h. One day-old males had the longest cumulative flight distance; the average cumulative flight distance and average cumulative flight time of males decreased with age. The maximum female flight distance was 8.21 km and the longest cumulative flight time was 7.4 h. Female flight distance tended to first increase, then decrease, with age. Three day-old females had the maximum cumulative flight distance. A two-way ANOVA of the average cumulative flight distance, average cumulative flight time, and average flight speed, did not detect a significant interaction between sex and age. Male flight speed was significantly slower at a temperature of 16 ℃. [Conclusion]  Male H. cunea are stronger fliers than females. Male flight ability gradually decreases with age, whereas that of females tends to increase up to 3 days of age, then decrease. Low temperatures reduce the flight speed of H. cunea males.