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斑翅果蝇求偶行为的研究
The courtship behavior of Drosophila suzukii (Diptera: Drosophilidae)
熊 焰,施 米,张金平,张 峰,李 萍,肖 春
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DOI:10.7679/j.issn.2095-1353.2023.015
作者单位:陇东学院生命科学与技术学院,庆阳 745000
中文关键词:斑翅果蝇;求偶行为;视觉因素;性信息素
英文关键词:spotted wing drosophila, courtship behavior, visual cues, sex pheromone
中文摘要:

【目的】 明确斑翅果蝇Drosophila suzuki求偶行为的特征及过程、视觉因素与信息素粗提物在求偶行为中的作用。【方法】 在室内条件下[温度(24 ± 1℃、相对湿度65% ± 5%,光周期14L∶10D],通过一系列的行为实验观察斑翅果蝇的求偶行为特征及过程。【结果】 雄虫的求偶特征为当雌雄虫相遇前或相遇时,雌虫腹部末端翘起,雄虫转向追逐雌虫且翅膀展开;当雌虫静止时,雄虫在雌虫前方展开翅膀并伴随翅膀振动,之后雄虫绕到雌虫侧面,使用前足触碰雌虫并很快返回雌虫前方展开翅膀,随后绕到雌虫尾部弯曲腹部试图进行交配。3日龄雌虫的求偶行为次数最多,为(127.4 ± 10.0)次,且求偶高峰期出现在进入光照期后的3-4 h时段内,为(59.2 ± 5.4)次。与单一因素相比,未交配雌虫卵巢粗提物与死亡虫体共同作用时能够显著激发雄虫的求偶行为。进一步观察结果显示,卵巢粗提物对斑翅果蝇未交配雄虫具有显著的引诱效果,但仅有卵巢粗提物出现时并不能显著增强雄虫求偶行为。【结论】 能引起斑翅果蝇雄虫求偶的化学物质(性信息素)存在斑翅果蝇雌虫卵巢中,视觉因素和性信息素联合作用才能显著增强雄虫的求偶行为。

英文摘要:

[Objectives]  To clarify the courtship behavior of Drosophila suzukii, the role of vision and crude pheromone extracts in courtship behavior. [Methods] A series of behavioral experiments were conducted under indoor conditions [temperature (24±1) ℃relative humidity 65%±5%photoperiod 14L︰10D] to determine the courting behavior characteristics of D. suzukii. [Results]  The courtship behavior characteristics of males are as follows: when the female and male meeet before or when they meet, the end of the female's abdomen is raised, males approached females, following them and performing a wing scissoring display ; When the female is statinary, the male spreads and vibrates in front of the female, then the male circles around the side of the female, touches the female with the front feet and quickly returning to his former position, then goes around the tail of the female and attempts to mate with his abdomen bent down and forward. The number of courtship behaviors performed per day by female 3 day-old instars (172.4 ± 10.0) was significantly higher than the number performed by younger or older instars , and the courtship peak period [(59.2±5.4)times] observed between 3 h and 4 h after the start of the light-period than during the rest of the light-period. Compared with a sigle factor, the combined action of the female body eluent and the dead body can significantly stimulate the male’s courtship behavior. Futher observatios showed that crude ovarian extracts had a significant attracting effect on unmated males of D. suzukii, but the presence of ovarian extracts alone did not significantly enhance courtship behavior of males. [Conclusion]  Pheromones in the ovaries of D. suzukii females, together with visual cues, play an important role in inducing male courtship behavior.

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