【目的】 为明确福建省草地贪夜蛾Spodoptera frugiperda田间种群对农业农村部推荐使用的主要防治药剂的敏感性。【方法】 采用浸叶法测定了南平建瓯、三明永安、福州福清和漳州长泰4个地区草地贪夜蛾田间种群对甲氨基阿维菌素苯甲酸盐（甲维盐）、乙基多杀菌素、茚虫威、氯虫苯甲酰胺、虫螨腈和虱螨脲6种具有较高防效的不同类型杀虫剂的敏感性，同时对比了长泰和建瓯春秋两季草地贪夜蛾田间种群对上述6种杀虫剂的敏感性。【结果】 不同地域的草地贪夜蛾对杀虫剂的敏感性存在显著差异，其中甲维盐对长泰、福清种群的LC₅₀值是建瓯、永安种群的2倍，茚虫威对长泰、福清种群的LC₅₀值是永安种群的3倍；草地贪夜蛾对杀虫剂的敏感性具有明显的季节性差异，其中氯虫苯甲酰胺和虫螨腈对长泰秋季种群的LC₅₀值分别是春季种群的0.20倍和2.26倍，甲维盐和茚虫威对建瓯秋季种群的LC₅₀值分别是春季种群的4.75倍和2.20倍；草地贪夜蛾田间种群在室内无药剂选择压力下连续饲养10代后，对虱螨脲、茚虫威和氯虫苯甲酰胺3种杀虫剂的敏感性提高最为显著，F₁代的LC₅₀值分别是F₁₀代的8.15倍、22.87倍和10.58倍。【结论】 福建省4个地区草地贪夜蛾田间种群对甲氨基阿维菌素苯甲酸盐（甲维盐）、乙基多杀菌素、茚虫威、氯虫苯甲酰胺、虫螨腈和虱螨脲较为敏感，长泰和建瓯春秋两季种群对氯虫苯甲酰胺、虫螨腈、茚虫威和虱螨脲的敏感性存在明显差异。

[Objectives]  To determine the sensitivity of Spodoptera frugiperda in Fujian province to six insecticides recommended by the Ministry of Agriculture and Rural Affairs. [Methods]  The leaf dip method was used to determine the lethal concentration 50 (LC₅₀) of six insecticides (emamectin benzoate, spinetoram, indoxacarb, chlorantraniliprole, clorfenapyr and lufenuron) to S. frugiperda, collected in Jian’ou county of Nanping city, Yong’an county of Sangming city, Fuqing county of Fuzhou city and Changtai county of Zhangzhou city. The LC₅₀ values of these six insecticides were also compared between specimens collected in Jian’ou and Changtai populations in spring and autumn. [Results]  The susceptibility of S. frugiperda to the insecticides tested varied significantly among populations. The LC₅₀ of emamectin benzoate in the Changtai and Fuqing populations was about twice than that in the Jian'ou and Yong'an populations, and the LC₅₀ of indoxacarb in the Changtai and Fuqing populations was about 3 times than that in the Yong'an population. The susceptibility of S. frugiperda to insecticides also varied between seasons. The LC₅₀ of chlorantraniliprole and clorfenapyr to the Changtai population was 0.20 times and 2.26 times, respectively, in autumn than that in spring, and the LC₅₀ of emamectin benzoate and indoxacarb to the Jian’ou population was 4.75 times and 2.20 times, respectively, in autumn than that in spring. Rearing ten successive generations without insecticide exposure caused the LC₅₀ values of lufenuron, indoxacarb and chlorantraniliprole to decrease 8.15, 22.87 and 10.58 fold, respectively, compared to the F₁ generation. [Conclusion]  Field populations of S. frugiperda in Fujian province are generally still sensitive to the six tested insecticides in 2020. In Changtai and Jian’ou, the sensitivity of S. frugiperda to chlorantraniliprole, clorfenapyr, indoxacarb, lufenuron differed significantly between spring and autumn.